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A twist defect mechanism for ATP-dependent translocation of nucleosomal DNA
As superfamily 2 (SF2)-type translocases, chromatin remodelers are expected to use an inchworm-type mechanism to walk along DNA. Yet how they move DNA around the histone core has not been clear. Here we show that a remodeler ATPase motor can shift large segments of DNA by changing the twist and leng...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031429/ https://www.ncbi.nlm.nih.gov/pubmed/29809147 http://dx.doi.org/10.7554/eLife.34100 |
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author | Winger, Jessica Nodelman, Ilana M Levendosky, Robert F Bowman, Gregory D |
author_facet | Winger, Jessica Nodelman, Ilana M Levendosky, Robert F Bowman, Gregory D |
author_sort | Winger, Jessica |
collection | PubMed |
description | As superfamily 2 (SF2)-type translocases, chromatin remodelers are expected to use an inchworm-type mechanism to walk along DNA. Yet how they move DNA around the histone core has not been clear. Here we show that a remodeler ATPase motor can shift large segments of DNA by changing the twist and length of nucleosomal DNA at superhelix location 2 (SHL2). Using canonical and variant 601 nucleosomes, we find that the Saccharomyces cerevisiae Chd1 remodeler decreased DNA twist at SHL2 in nucleotide-free and ADP-bound states, and increased twist with transition state analogs. These differences in DNA twist allow the open state of the ATPase to pull in ~1 base pair (bp) by stabilizing a small DNA bulge, and closure of the ATPase to shift the DNA bulge toward the dyad. We propose that such formation and elimination of twist defects underlie the mechanism of nucleosome sliding by CHD-, ISWI-, and SWI/SNF-type remodelers. |
format | Online Article Text |
id | pubmed-6031429 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-60314292018-07-06 A twist defect mechanism for ATP-dependent translocation of nucleosomal DNA Winger, Jessica Nodelman, Ilana M Levendosky, Robert F Bowman, Gregory D eLife Biochemistry and Chemical Biology As superfamily 2 (SF2)-type translocases, chromatin remodelers are expected to use an inchworm-type mechanism to walk along DNA. Yet how they move DNA around the histone core has not been clear. Here we show that a remodeler ATPase motor can shift large segments of DNA by changing the twist and length of nucleosomal DNA at superhelix location 2 (SHL2). Using canonical and variant 601 nucleosomes, we find that the Saccharomyces cerevisiae Chd1 remodeler decreased DNA twist at SHL2 in nucleotide-free and ADP-bound states, and increased twist with transition state analogs. These differences in DNA twist allow the open state of the ATPase to pull in ~1 base pair (bp) by stabilizing a small DNA bulge, and closure of the ATPase to shift the DNA bulge toward the dyad. We propose that such formation and elimination of twist defects underlie the mechanism of nucleosome sliding by CHD-, ISWI-, and SWI/SNF-type remodelers. eLife Sciences Publications, Ltd 2018-05-29 /pmc/articles/PMC6031429/ /pubmed/29809147 http://dx.doi.org/10.7554/eLife.34100 Text en © 2018, Winger et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Biochemistry and Chemical Biology Winger, Jessica Nodelman, Ilana M Levendosky, Robert F Bowman, Gregory D A twist defect mechanism for ATP-dependent translocation of nucleosomal DNA |
title | A twist defect mechanism for ATP-dependent translocation of nucleosomal DNA |
title_full | A twist defect mechanism for ATP-dependent translocation of nucleosomal DNA |
title_fullStr | A twist defect mechanism for ATP-dependent translocation of nucleosomal DNA |
title_full_unstemmed | A twist defect mechanism for ATP-dependent translocation of nucleosomal DNA |
title_short | A twist defect mechanism for ATP-dependent translocation of nucleosomal DNA |
title_sort | twist defect mechanism for atp-dependent translocation of nucleosomal dna |
topic | Biochemistry and Chemical Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031429/ https://www.ncbi.nlm.nih.gov/pubmed/29809147 http://dx.doi.org/10.7554/eLife.34100 |
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