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A twist defect mechanism for ATP-dependent translocation of nucleosomal DNA

As superfamily 2 (SF2)-type translocases, chromatin remodelers are expected to use an inchworm-type mechanism to walk along DNA. Yet how they move DNA around the histone core has not been clear. Here we show that a remodeler ATPase motor can shift large segments of DNA by changing the twist and leng...

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Detalles Bibliográficos
Autores principales: Winger, Jessica, Nodelman, Ilana M, Levendosky, Robert F, Bowman, Gregory D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031429/
https://www.ncbi.nlm.nih.gov/pubmed/29809147
http://dx.doi.org/10.7554/eLife.34100
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author Winger, Jessica
Nodelman, Ilana M
Levendosky, Robert F
Bowman, Gregory D
author_facet Winger, Jessica
Nodelman, Ilana M
Levendosky, Robert F
Bowman, Gregory D
author_sort Winger, Jessica
collection PubMed
description As superfamily 2 (SF2)-type translocases, chromatin remodelers are expected to use an inchworm-type mechanism to walk along DNA. Yet how they move DNA around the histone core has not been clear. Here we show that a remodeler ATPase motor can shift large segments of DNA by changing the twist and length of nucleosomal DNA at superhelix location 2 (SHL2). Using canonical and variant 601 nucleosomes, we find that the Saccharomyces cerevisiae Chd1 remodeler decreased DNA twist at SHL2 in nucleotide-free and ADP-bound states, and increased twist with transition state analogs. These differences in DNA twist allow the open state of the ATPase to pull in ~1 base pair (bp) by stabilizing a small DNA bulge, and closure of the ATPase to shift the DNA bulge toward the dyad. We propose that such formation and elimination of twist defects underlie the mechanism of nucleosome sliding by CHD-, ISWI-, and SWI/SNF-type remodelers.
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spelling pubmed-60314292018-07-06 A twist defect mechanism for ATP-dependent translocation of nucleosomal DNA Winger, Jessica Nodelman, Ilana M Levendosky, Robert F Bowman, Gregory D eLife Biochemistry and Chemical Biology As superfamily 2 (SF2)-type translocases, chromatin remodelers are expected to use an inchworm-type mechanism to walk along DNA. Yet how they move DNA around the histone core has not been clear. Here we show that a remodeler ATPase motor can shift large segments of DNA by changing the twist and length of nucleosomal DNA at superhelix location 2 (SHL2). Using canonical and variant 601 nucleosomes, we find that the Saccharomyces cerevisiae Chd1 remodeler decreased DNA twist at SHL2 in nucleotide-free and ADP-bound states, and increased twist with transition state analogs. These differences in DNA twist allow the open state of the ATPase to pull in ~1 base pair (bp) by stabilizing a small DNA bulge, and closure of the ATPase to shift the DNA bulge toward the dyad. We propose that such formation and elimination of twist defects underlie the mechanism of nucleosome sliding by CHD-, ISWI-, and SWI/SNF-type remodelers. eLife Sciences Publications, Ltd 2018-05-29 /pmc/articles/PMC6031429/ /pubmed/29809147 http://dx.doi.org/10.7554/eLife.34100 Text en © 2018, Winger et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biochemistry and Chemical Biology
Winger, Jessica
Nodelman, Ilana M
Levendosky, Robert F
Bowman, Gregory D
A twist defect mechanism for ATP-dependent translocation of nucleosomal DNA
title A twist defect mechanism for ATP-dependent translocation of nucleosomal DNA
title_full A twist defect mechanism for ATP-dependent translocation of nucleosomal DNA
title_fullStr A twist defect mechanism for ATP-dependent translocation of nucleosomal DNA
title_full_unstemmed A twist defect mechanism for ATP-dependent translocation of nucleosomal DNA
title_short A twist defect mechanism for ATP-dependent translocation of nucleosomal DNA
title_sort twist defect mechanism for atp-dependent translocation of nucleosomal dna
topic Biochemistry and Chemical Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031429/
https://www.ncbi.nlm.nih.gov/pubmed/29809147
http://dx.doi.org/10.7554/eLife.34100
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