Optimization of sample preparation and green color imaging using the mNeonGreen fluorescent protein in bacterial cells for photoactivated localization microscopy

mNeonGreen fluorescent protein is capable of photo-switching, hence in principle applicable for super-resolution imaging. However, difficult-to-control blinking kinetics that lead to simultaneous emission of multiple nearby mNeonGreen molecules impedes its use for PALM. Here, we determined the on- a...

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Detalles Bibliográficos
Autores principales: Stockmar, Iris, Feddersen, Helge, Cramer, Kimberly, Gruber, Stephan, Jung, Kirsten, Bramkamp, Marc, Shin, Jae Yen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031688/
https://www.ncbi.nlm.nih.gov/pubmed/29973667
http://dx.doi.org/10.1038/s41598-018-28472-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031688/
https://www.ncbi.nlm.nih.gov/pubmed/29973667
http://dx.doi.org/10.1038/s41598-018-28472-0

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