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Isobavachalcone from Angelica keiskei Inhibits Adipogenesis and Prevents Lipid Accumulation

We isolated isobavachalcone (IBC) from Angelica keiskei (AK) as an anti-obesity component. IBC dose-dependently inhibited 3T3-L1 adipocyte differentiation by down-regulating adipogenic factors. At the mitotic clonal expansion stage (MCE), IBC caused cell cycle arrest in G0/G1 with decreased expressi...

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Detalles Bibliográficos
Autores principales: Lee, Hyejin, Li, Hua, Kweon, Minson, Choi, Youngsook, Kim, Min Jung, Ryu, Jae-Ha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032101/
https://www.ncbi.nlm.nih.gov/pubmed/29882838
http://dx.doi.org/10.3390/ijms19061693
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author Lee, Hyejin
Li, Hua
Kweon, Minson
Choi, Youngsook
Kim, Min Jung
Ryu, Jae-Ha
author_facet Lee, Hyejin
Li, Hua
Kweon, Minson
Choi, Youngsook
Kim, Min Jung
Ryu, Jae-Ha
author_sort Lee, Hyejin
collection PubMed
description We isolated isobavachalcone (IBC) from Angelica keiskei (AK) as an anti-obesity component. IBC dose-dependently inhibited 3T3-L1 adipocyte differentiation by down-regulating adipogenic factors. At the mitotic clonal expansion stage (MCE), IBC caused cell cycle arrest in G0/G1 with decreased expression of cell cycle-regulating proteins. IBC also inhibited autophagic flux by inducing intracellular accumulation of LC3B and SQSTM1/p62 proteins while decreasing expression levels of regulating factors for autophagy initiation. In parallel with the inhibition of adipocyte differentiation, IBC decreased intrahepatic fat deposits and rescued the liver steatosis in high fat cholesterol diet-fed zebrafish. In this study, we found that IBC isolated from AK suppresses mitotic clonal expansion and autophagy flux of adipocytes and also shows anti-obesity activity in a high cholesterol-diet zebrafish model by decreasing intrahepatic fat deposits. These results suggest that IBC could be a leading pharmacological compound for the development of anti-obesity drugs.
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spelling pubmed-60321012018-07-13 Isobavachalcone from Angelica keiskei Inhibits Adipogenesis and Prevents Lipid Accumulation Lee, Hyejin Li, Hua Kweon, Minson Choi, Youngsook Kim, Min Jung Ryu, Jae-Ha Int J Mol Sci Article We isolated isobavachalcone (IBC) from Angelica keiskei (AK) as an anti-obesity component. IBC dose-dependently inhibited 3T3-L1 adipocyte differentiation by down-regulating adipogenic factors. At the mitotic clonal expansion stage (MCE), IBC caused cell cycle arrest in G0/G1 with decreased expression of cell cycle-regulating proteins. IBC also inhibited autophagic flux by inducing intracellular accumulation of LC3B and SQSTM1/p62 proteins while decreasing expression levels of regulating factors for autophagy initiation. In parallel with the inhibition of adipocyte differentiation, IBC decreased intrahepatic fat deposits and rescued the liver steatosis in high fat cholesterol diet-fed zebrafish. In this study, we found that IBC isolated from AK suppresses mitotic clonal expansion and autophagy flux of adipocytes and also shows anti-obesity activity in a high cholesterol-diet zebrafish model by decreasing intrahepatic fat deposits. These results suggest that IBC could be a leading pharmacological compound for the development of anti-obesity drugs. MDPI 2018-06-06 /pmc/articles/PMC6032101/ /pubmed/29882838 http://dx.doi.org/10.3390/ijms19061693 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Hyejin
Li, Hua
Kweon, Minson
Choi, Youngsook
Kim, Min Jung
Ryu, Jae-Ha
Isobavachalcone from Angelica keiskei Inhibits Adipogenesis and Prevents Lipid Accumulation
title Isobavachalcone from Angelica keiskei Inhibits Adipogenesis and Prevents Lipid Accumulation
title_full Isobavachalcone from Angelica keiskei Inhibits Adipogenesis and Prevents Lipid Accumulation
title_fullStr Isobavachalcone from Angelica keiskei Inhibits Adipogenesis and Prevents Lipid Accumulation
title_full_unstemmed Isobavachalcone from Angelica keiskei Inhibits Adipogenesis and Prevents Lipid Accumulation
title_short Isobavachalcone from Angelica keiskei Inhibits Adipogenesis and Prevents Lipid Accumulation
title_sort isobavachalcone from angelica keiskei inhibits adipogenesis and prevents lipid accumulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032101/
https://www.ncbi.nlm.nih.gov/pubmed/29882838
http://dx.doi.org/10.3390/ijms19061693
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