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An improvised one-step sucrose cushion ultracentrifugation method for exosome isolation from culture supernatants of mesenchymal stem cells

BACKGROUND: Exosomes are nanovesicles (30–120 nm) of endosomal origin. These exosomes contain various functional proteins and RNAs that could be used for therapeutic purposes. Currently, having a standard method for exosome isolation retaining its biological properties with increased yield and purit...

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Detalles Bibliográficos
Autores principales: Gupta, Suchi, Rawat, Sonali, Arora, Vivek, Kottarath, Sarat Kumar, Dinda, Amit Kumar, Vaishnav, Pradeep Kumar, Nayak, Baibaswata, Mohanty, Sujata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6033286/
https://www.ncbi.nlm.nih.gov/pubmed/29973270
http://dx.doi.org/10.1186/s13287-018-0923-0