Genome-Wide Determination of Gene Essentiality by Transposon Insertion Sequencing in Yeast Pichia pastoris

In many prokaryotes but limited eukaryotic species, the combination of transposon mutagenesis and high-throughput sequencing has greatly accelerated the identification of essential genes. Here we successfully applied this technique to the methylotrophic yeast Pichia pastoris and classified its condi...

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Detalles Bibliográficos
Autores principales: Zhu, Jinxiang, Gong, Ruiqing, Zhu, Qiaoyun, He, Qiulin, Xu, Ning, Xu, Yichun, Cai, Menghao, Zhou, Xiangshan, Zhang, Yuanxing, Zhou, Mian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6033949/
https://www.ncbi.nlm.nih.gov/pubmed/29976927
http://dx.doi.org/10.1038/s41598-018-28217-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6033949/
https://www.ncbi.nlm.nih.gov/pubmed/29976927
http://dx.doi.org/10.1038/s41598-018-28217-z

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