Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing

RNA-based drugs depend on chemical modifications to increase potency and to decrease immunogenicity in vivo. Chemical modification will likely improve the guide RNAs involved in CRISPR-Cas9-based therapeutics as well. Cas9 orthologs are RNA-guided microbial effectors that cleave DNA. Here, we explor...

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Detalles Bibliográficos
Autores principales: Mir, Aamir, Alterman, Julia F., Hassler, Matthew R., Debacker, Alexandre J., Hudgens, Edward, Echeverria, Dimas, Brodsky, Michael H., Khvorova, Anastasia, Watts, Jonathan K., Sontheimer, Erik J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6035171/
https://www.ncbi.nlm.nih.gov/pubmed/29980686
http://dx.doi.org/10.1038/s41467-018-05073-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6035171/
https://www.ncbi.nlm.nih.gov/pubmed/29980686
http://dx.doi.org/10.1038/s41467-018-05073-z

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