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Enzyme Architecture: Amino Acid Side-Chains That Function To Optimize the Basicity of the Active Site Glutamate of Triosephosphate Isomerase

[Image: see text] We report pH rate profiles for k(cat) and K(m) for the isomerization reaction of glyceraldehyde 3-phosphate catalyzed by wildtype triosephosphate isomerase (TIM) from three organisms and by ten mutants of TIM; and, for K(i) for inhibition of this reaction by phosphoglycolate triani...

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Detalles Bibliográficos
Autores principales: Zhai, Xiang, Reinhardt, Christopher J., Malabanan, M. Merced, Amyes, Tina L., Richard, John P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037162/
https://www.ncbi.nlm.nih.gov/pubmed/29862813
http://dx.doi.org/10.1021/jacs.8b04367