Single-cell cloning enables the selection of more productive Drosophila melanogaster S2 cells for recombinant protein expression

The generation of monoclonal cell lines is an important early process development step for recombinant protein production. Although single-cell cloning is an established method in mammalian cell lines, straightforward protocols are not yet available for insect cells. We describe a new method for the...

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Detalles Bibliográficos
Autores principales: Zitzmann, Jan, Schreiber, Christine, Eichmann, Joel, Bilz, Roberto Otmar, Salzig, Denise, Weidner, Tobias, Czermak, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037645/
https://www.ncbi.nlm.nih.gov/pubmed/29998071
http://dx.doi.org/10.1016/j.btre.2018.e00272

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037645/
https://www.ncbi.nlm.nih.gov/pubmed/29998071
http://dx.doi.org/10.1016/j.btre.2018.e00272

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