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Single-cell cloning enables the selection of more productive Drosophila melanogaster S2 cells for recombinant protein expression
The generation of monoclonal cell lines is an important early process development step for recombinant protein production. Although single-cell cloning is an established method in mammalian cell lines, straightforward protocols are not yet available for insect cells. We describe a new method for the...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037645/ https://www.ncbi.nlm.nih.gov/pubmed/29998071 http://dx.doi.org/10.1016/j.btre.2018.e00272 |