Heterogeneity in tumor chromatin-doxorubicin binding revealed by in vivo fluorescence lifetime imaging confocal endomicroscopy

We present an approach to quantify drug–target engagement using in vivo fluorescence endomicroscopy, validated with in vitro measurements. Doxorubicin binding to chromatin changes the fluorescence lifetime of histone-GFP fusions that we measure in vivo at single-cell resolution using a confocal lapa...

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Detalles Bibliográficos
Autores principales: Sparks, Hugh, Kondo, Hiroshi, Hooper, Steven, Munro, Ian, Kennedy, Gordon, Dunsby, Christopher, French, Paul, Sahai, Erik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037736/
https://www.ncbi.nlm.nih.gov/pubmed/29985394
http://dx.doi.org/10.1038/s41467-018-04820-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037736/
https://www.ncbi.nlm.nih.gov/pubmed/29985394
http://dx.doi.org/10.1038/s41467-018-04820-6

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