Optimized paired‐sgRNA/Cas9 cloning and expression cassette triggers high‐efficiency multiplex genome editing in kiwifruit

Kiwifruit is an important fruit crop; however, technologies for its functional genomic and molecular improvement are limited. The clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein (Cas) system has been successfully applied to genetic improvement in many cr...

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Detalles Bibliográficos
Autores principales: Wang, Zupeng, Wang, Shuaibin, Li, Dawei, Zhang, Qiong, Li, Li, Zhong, Caihong, Liu, Yifei, Huang, Hongwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6041439/
https://www.ncbi.nlm.nih.gov/pubmed/29331077
http://dx.doi.org/10.1111/pbi.12884

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6041439/
https://www.ncbi.nlm.nih.gov/pubmed/29331077
http://dx.doi.org/10.1111/pbi.12884

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