Elimination of PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers

BACKGROUND: RNA-seq and small RNA-seq are powerful, quantitative tools to study gene regulation and function. Common high-throughput sequencing methods rely on polymerase chain reaction (PCR) to expand the starting material, but not every molecule amplifies equally, causing some to be overrepresente...

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Detalles Bibliográficos
Autores principales: Fu, Yu, Wu, Pei-Hsuan, Beane, Timothy, Zamore, Phillip D., Weng, Zhiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044086/
https://www.ncbi.nlm.nih.gov/pubmed/30001700
http://dx.doi.org/10.1186/s12864-018-4933-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044086/
https://www.ncbi.nlm.nih.gov/pubmed/30001700
http://dx.doi.org/10.1186/s12864-018-4933-1

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