Laboratory evolution of a glucose-phosphorylation-deficient, arabinose-fermenting S. cerevisiae strain reveals mutations in GAL2 that enable glucose-insensitive l-arabinose uptake

Cas9-assisted genome editing was used to construct an engineered glucose-phosphorylation-negative S. cerevisiae strain, expressing the Lactobacillus plantaruml-arabinose pathway and the Penicillium chrysogenum transporter PcAraT. This strain, which showed a growth rate of 0.26 h(−1) on l-arabinose i...

Descripción completa

Detalles Bibliográficos
Autores principales: Verhoeven, Maarten D, Bracher, Jasmine M, Nijland, Jeroen G, Bouwknegt, Jonna, Daran, Jean-Marc G, Driessen, Arnold J M, van Maris, Antonius J A, Pronk, Jack T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044391/
https://www.ncbi.nlm.nih.gov/pubmed/29860442
http://dx.doi.org/10.1093/femsyr/foy062

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044391/
https://www.ncbi.nlm.nih.gov/pubmed/29860442
http://dx.doi.org/10.1093/femsyr/foy062

Ejemplares similares