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Laboratory evolution of a glucose-phosphorylation-deficient, arabinose-fermenting S. cerevisiae strain reveals mutations in GAL2 that enable glucose-insensitive l-arabinose uptake

Cas9-assisted genome editing was used to construct an engineered glucose-phosphorylation-negative S. cerevisiae strain, expressing the Lactobacillus plantaruml-arabinose pathway and the Penicillium chrysogenum transporter PcAraT. This strain, which showed a growth rate of 0.26 h(−1) on l-arabinose i...

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Detalles Bibliográficos
Autores principales:	Verhoeven, Maarten D, Bracher, Jasmine M, Nijland, Jeroen G, Bouwknegt, Jonna, Daran, Jean-Marc G, Driessen, Arnold J M, van Maris, Antonius J A, Pronk, Jack T
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2018
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044391/ https://www.ncbi.nlm.nih.gov/pubmed/29860442 http://dx.doi.org/10.1093/femsyr/foy062

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