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Post-Translational Sortase-Mediated Attachment of High-Strength Force Spectroscopy Handles

[Image: see text] Single-molecule force spectroscopy greatly benefits from site-specific surface immobilization and specific probing with a functionalized cantilever. Here, we describe a streamlined approach to such experiments by covalently attaching mechanically stable receptors onto proteins of i...

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Detalles Bibliográficos
Autores principales: Durner, Ellis, Ott, Wolfgang, Nash, Michael A., Gaub, Hermann E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044863/
https://www.ncbi.nlm.nih.gov/pubmed/30023682
http://dx.doi.org/10.1021/acsomega.7b00478