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Self-Assembly Mechanism of a Peptide-Based Drug Delivery Vehicle

[Image: see text] We report the mechanism of the concentration-dependent self-assembly of a tetrapeptide. Peptide Boc-Trp-Leu-Trp-Leu-OMe self-assembles to form discrete nanospheres at a low concentration. Tryptophan side chains point outwards of the nanospheres while leucine side chains point towar...

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Autores principales: Pandit, Gopal, Roy, Karabi, Agarwal, Umang, Chatterjee, Sunanda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045401/
https://www.ncbi.nlm.nih.gov/pubmed/30023862
http://dx.doi.org/10.1021/acsomega.7b01871
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author Pandit, Gopal
Roy, Karabi
Agarwal, Umang
Chatterjee, Sunanda
author_facet Pandit, Gopal
Roy, Karabi
Agarwal, Umang
Chatterjee, Sunanda
author_sort Pandit, Gopal
collection PubMed
description [Image: see text] We report the mechanism of the concentration-dependent self-assembly of a tetrapeptide. Peptide Boc-Trp-Leu-Trp-Leu-OMe self-assembles to form discrete nanospheres at a low concentration. Tryptophan side chains point outwards of the nanospheres while leucine side chains point towards the core of the nanospheres. The nanospheres fuse together to become microspheres with the increase in the peptide concentration. At higher concentrations of the peptide, the microspheres start clustering. This is stabilized by the aromatic interactions between the side chains of the tryptophan residues that cover the outer surface of the peptide microspheres. In addition to behaving like the conventional hollow sphere-based drug delivery vehicles which entraps the drug and performs stimuli-responsive release, this prototype can interact, stabilize, and intercalate hydrophobic dye carboxyfluorescein and anti-cancer drug curcumin even on the surface through aromatic interactions. The dye/drug can be released in acidic pH and in the presence of physiologically relevant ions such as potassium.
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spelling pubmed-60454012018-07-16 Self-Assembly Mechanism of a Peptide-Based Drug Delivery Vehicle Pandit, Gopal Roy, Karabi Agarwal, Umang Chatterjee, Sunanda ACS Omega [Image: see text] We report the mechanism of the concentration-dependent self-assembly of a tetrapeptide. Peptide Boc-Trp-Leu-Trp-Leu-OMe self-assembles to form discrete nanospheres at a low concentration. Tryptophan side chains point outwards of the nanospheres while leucine side chains point towards the core of the nanospheres. The nanospheres fuse together to become microspheres with the increase in the peptide concentration. At higher concentrations of the peptide, the microspheres start clustering. This is stabilized by the aromatic interactions between the side chains of the tryptophan residues that cover the outer surface of the peptide microspheres. In addition to behaving like the conventional hollow sphere-based drug delivery vehicles which entraps the drug and performs stimuli-responsive release, this prototype can interact, stabilize, and intercalate hydrophobic dye carboxyfluorescein and anti-cancer drug curcumin even on the surface through aromatic interactions. The dye/drug can be released in acidic pH and in the presence of physiologically relevant ions such as potassium. American Chemical Society 2018-03-15 /pmc/articles/PMC6045401/ /pubmed/30023862 http://dx.doi.org/10.1021/acsomega.7b01871 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Pandit, Gopal
Roy, Karabi
Agarwal, Umang
Chatterjee, Sunanda
Self-Assembly Mechanism of a Peptide-Based Drug Delivery Vehicle
title Self-Assembly Mechanism of a Peptide-Based Drug Delivery Vehicle
title_full Self-Assembly Mechanism of a Peptide-Based Drug Delivery Vehicle
title_fullStr Self-Assembly Mechanism of a Peptide-Based Drug Delivery Vehicle
title_full_unstemmed Self-Assembly Mechanism of a Peptide-Based Drug Delivery Vehicle
title_short Self-Assembly Mechanism of a Peptide-Based Drug Delivery Vehicle
title_sort self-assembly mechanism of a peptide-based drug delivery vehicle
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045401/
https://www.ncbi.nlm.nih.gov/pubmed/30023862
http://dx.doi.org/10.1021/acsomega.7b01871
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