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Quantification of sugars and organic acids in tomato fruits

Sugar and organic acid contents are major factors for tomato fruit flavour and are important breeding traits. Here we provide an improved protocol for accurate quantification of the main sugars, glucose and fructose, and the organic acids, citric acid and malic acid, present in tomato. The tomato ex...

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Autores principales: Agius, Carlos, von Tucher, Sabine, Poppenberger, Brigitte, Rozhon, Wilfried
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6046607/
https://www.ncbi.nlm.nih.gov/pubmed/30023316
http://dx.doi.org/10.1016/j.mex.2018.05.014
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author Agius, Carlos
von Tucher, Sabine
Poppenberger, Brigitte
Rozhon, Wilfried
author_facet Agius, Carlos
von Tucher, Sabine
Poppenberger, Brigitte
Rozhon, Wilfried
author_sort Agius, Carlos
collection PubMed
description Sugar and organic acid contents are major factors for tomato fruit flavour and are important breeding traits. Here we provide an improved protocol for accurate quantification of the main sugars, glucose and fructose, and the organic acids, citric acid and malic acid, present in tomato. The tomato extract is spiked with lactose and tricarballylic acid as internal standards and loaded onto a NH2 solid phase extraction (SPE) column. The sugars appear in the flow-through and are subsequently analysed by HPLC using a Nucleodur NH2 column and a refractive index detector. The organic acids bind to the SPE column and are eluted with 400 mM phosphoric acid. For analysis, the organic acids are separated by HPLC using a Nucleodur C18ec column and detected by UV absorption at 210 nm. The method shows excellent inter-day and intra-day reproducibility for glucose, fructose and citric acid with standard deviations of 1–5%. Quantification of citric acid by HPLC and GC–MS showed perfect agreement with a deviation of less than 3%. • Simple method for quantification of glucose, fructose, citric acid and malic acid in tomato. • Efficient removal of interfering compounds by solid phase extraction. • High intra and inter-day reproducibility.
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spelling pubmed-60466072018-07-18 Quantification of sugars and organic acids in tomato fruits Agius, Carlos von Tucher, Sabine Poppenberger, Brigitte Rozhon, Wilfried MethodsX Agricultural and Biological Science Sugar and organic acid contents are major factors for tomato fruit flavour and are important breeding traits. Here we provide an improved protocol for accurate quantification of the main sugars, glucose and fructose, and the organic acids, citric acid and malic acid, present in tomato. The tomato extract is spiked with lactose and tricarballylic acid as internal standards and loaded onto a NH2 solid phase extraction (SPE) column. The sugars appear in the flow-through and are subsequently analysed by HPLC using a Nucleodur NH2 column and a refractive index detector. The organic acids bind to the SPE column and are eluted with 400 mM phosphoric acid. For analysis, the organic acids are separated by HPLC using a Nucleodur C18ec column and detected by UV absorption at 210 nm. The method shows excellent inter-day and intra-day reproducibility for glucose, fructose and citric acid with standard deviations of 1–5%. Quantification of citric acid by HPLC and GC–MS showed perfect agreement with a deviation of less than 3%. • Simple method for quantification of glucose, fructose, citric acid and malic acid in tomato. • Efficient removal of interfering compounds by solid phase extraction. • High intra and inter-day reproducibility. Elsevier 2018-05-25 /pmc/articles/PMC6046607/ /pubmed/30023316 http://dx.doi.org/10.1016/j.mex.2018.05.014 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Agricultural and Biological Science
Agius, Carlos
von Tucher, Sabine
Poppenberger, Brigitte
Rozhon, Wilfried
Quantification of sugars and organic acids in tomato fruits
title Quantification of sugars and organic acids in tomato fruits
title_full Quantification of sugars and organic acids in tomato fruits
title_fullStr Quantification of sugars and organic acids in tomato fruits
title_full_unstemmed Quantification of sugars and organic acids in tomato fruits
title_short Quantification of sugars and organic acids in tomato fruits
title_sort quantification of sugars and organic acids in tomato fruits
topic Agricultural and Biological Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6046607/
https://www.ncbi.nlm.nih.gov/pubmed/30023316
http://dx.doi.org/10.1016/j.mex.2018.05.014
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