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PBMC fixation and processing for Chromium single-cell RNA sequencing

BACKGROUND: Interest in single-cell transcriptomic analysis is growing rapidly, especially for profiling rare or heterogeneous populations of cells. In almost all reported works investigators have used live cells, which introduces cell stress during preparation and hinders complex study designs. Rec...

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Detalles Bibliográficos
Autores principales: Chen, Jinguo, Cheung, Foo, Shi, Rongye, Zhou, Huizhi, Lu, Wenrui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6050658/
https://www.ncbi.nlm.nih.gov/pubmed/30016977
http://dx.doi.org/10.1186/s12967-018-1578-4