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‘Read–through marking’ reveals differential nucleotide composition of read-through and truncated cDNAs in iCLIP
We established a modified iCLIP protocol, called ‘read-through marking’, which facilitates the detection of cDNAs that have not been truncated upon encountering the RNA–peptide complex during reverse transcription (read-through cDNAs). A large proportion of these cDNAs would be undesirable in an iCL...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
F1000 Research Limited
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051193/ https://www.ncbi.nlm.nih.gov/pubmed/30057947 http://dx.doi.org/10.12688/wellcomeopenres.14663.1 |