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Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides

Group B Streptococcus (GBS) is a perinatal pathogen and an emerging cause of disease in adults. Culture-independent GBS detection relies on polymerase chain reaction (PCR) of conserved genes, including sip. We demonstrate suboptimal sensitivity of the existing sip PCR strategy and validate an improv...

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Detalles Bibliográficos
Autores principales: Khatami, Ameneh, Randis, Tara M, Chamby, Anna, Hooven, Thomas A, Gegick, Margaret, Suzman, Evan, A’Hearn-Thomas, Brady, Steenhoff, Andrew P, Ratner, Adam J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051451/
https://www.ncbi.nlm.nih.gov/pubmed/30038931
http://dx.doi.org/10.1093/ofid/ofy164