Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides

Group B Streptococcus (GBS) is a perinatal pathogen and an emerging cause of disease in adults. Culture-independent GBS detection relies on polymerase chain reaction (PCR) of conserved genes, including sip. We demonstrate suboptimal sensitivity of the existing sip PCR strategy and validate an improv...

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Autores principales: Khatami, Ameneh, Randis, Tara M, Chamby, Anna, Hooven, Thomas A, Gegick, Margaret, Suzman, Evan, A’Hearn-Thomas, Brady, Steenhoff, Andrew P, Ratner, Adam J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051451/
https://www.ncbi.nlm.nih.gov/pubmed/30038931
http://dx.doi.org/10.1093/ofid/ofy164
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author Khatami, Ameneh
Randis, Tara M
Chamby, Anna
Hooven, Thomas A
Gegick, Margaret
Suzman, Evan
A’Hearn-Thomas, Brady
Steenhoff, Andrew P
Ratner, Adam J
author_facet Khatami, Ameneh
Randis, Tara M
Chamby, Anna
Hooven, Thomas A
Gegick, Margaret
Suzman, Evan
A’Hearn-Thomas, Brady
Steenhoff, Andrew P
Ratner, Adam J
author_sort Khatami, Ameneh
collection PubMed
description Group B Streptococcus (GBS) is a perinatal pathogen and an emerging cause of disease in adults. Culture-independent GBS detection relies on polymerase chain reaction (PCR) of conserved genes, including sip. We demonstrate suboptimal sensitivity of the existing sip PCR strategy and validate an improved method based on consensus sequences from >100 GBS genomes.
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spelling pubmed-60514512018-07-23 Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides Khatami, Ameneh Randis, Tara M Chamby, Anna Hooven, Thomas A Gegick, Margaret Suzman, Evan A’Hearn-Thomas, Brady Steenhoff, Andrew P Ratner, Adam J Open Forum Infect Dis Brief Report Group B Streptococcus (GBS) is a perinatal pathogen and an emerging cause of disease in adults. Culture-independent GBS detection relies on polymerase chain reaction (PCR) of conserved genes, including sip. We demonstrate suboptimal sensitivity of the existing sip PCR strategy and validate an improved method based on consensus sequences from >100 GBS genomes. Oxford University Press 2018-07-07 /pmc/articles/PMC6051451/ /pubmed/30038931 http://dx.doi.org/10.1093/ofid/ofy164 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Brief Report
Khatami, Ameneh
Randis, Tara M
Chamby, Anna
Hooven, Thomas A
Gegick, Margaret
Suzman, Evan
A’Hearn-Thomas, Brady
Steenhoff, Andrew P
Ratner, Adam J
Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides
title Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides
title_full Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides
title_fullStr Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides
title_full_unstemmed Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides
title_short Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides
title_sort improving the sensitivity of real-time pcr detection of group b streptococcus using consensus sequence-derived oligonucleotides
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6051451/
https://www.ncbi.nlm.nih.gov/pubmed/30038931
http://dx.doi.org/10.1093/ofid/ofy164
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