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Homologous alignment cloning: a rapid, flexible and highly efficient general molecular cloning method

Homologous alignment cloning (HAC) is a rapid method of molecular cloning that facilitates low-cost, highly efficient cloning of polymerase chain reaction products into any plasmid vector in approximately 2 min. HAC facilitates insert integration due to a sequence alignment strategy, by way of short...

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Detalles Bibliográficos
Autores principales: Tan, Lendl, Strong, Emily J., Woods, Kyra, West, Nicholas P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054264/
https://www.ncbi.nlm.nih.gov/pubmed/30038856
http://dx.doi.org/10.7717/peerj.5146