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Homologous alignment cloning: a rapid, flexible and highly efficient general molecular cloning method
Homologous alignment cloning (HAC) is a rapid method of molecular cloning that facilitates low-cost, highly efficient cloning of polymerase chain reaction products into any plasmid vector in approximately 2 min. HAC facilitates insert integration due to a sequence alignment strategy, by way of short...
Autores principales: | Tan, Lendl, Strong, Emily J., Woods, Kyra, West, Nicholas P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054264/ https://www.ncbi.nlm.nih.gov/pubmed/30038856 http://dx.doi.org/10.7717/peerj.5146 |
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