Programmable DNA repair with CRISPRa/i enhanced homology-directed repair efficiency with a single Cas9

CRISPR systems have been proven as versatile tools for site-specific genome engineering in mammalian species. During the gene editing processes, these RNA-guide nucleases introduce DNA double strand breaks (DSBs), in which non-homologous DNA end joining (NHEJ) dominates the DNA repair pathway, limit...

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Detalles Bibliográficos
Autores principales: Ye, Lupeng, Wang, Chengkun, Hong, Lingjuan, Sun, Ninghe, Chen, Danyang, Chen, Sidi, Han, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056518/
https://www.ncbi.nlm.nih.gov/pubmed/30062046
http://dx.doi.org/10.1038/s41421-018-0049-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056518/
https://www.ncbi.nlm.nih.gov/pubmed/30062046
http://dx.doi.org/10.1038/s41421-018-0049-7

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