A high-throughput imaging and nuclear segmentation analysis protocol for cleared 3D culture models

Imaging and subsequent segmentation analysis in three-dimensional (3D) culture models are complicated by the light scattering that occurs when collecting fluorescent signal through multiple cell and extracellular matrix layers. For 3D cell culture models to be usable for drug discovery, effective an...

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Detalles Bibliográficos
Autores principales: Boutin, Molly E., Voss, Ty C., Titus, Steven A., Cruz-Gutierrez, Kennie, Michael, Sam, Ferrer, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057966/
https://www.ncbi.nlm.nih.gov/pubmed/30042482
http://dx.doi.org/10.1038/s41598-018-29169-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057966/
https://www.ncbi.nlm.nih.gov/pubmed/30042482
http://dx.doi.org/10.1038/s41598-018-29169-0

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