Cargando…
A high-throughput imaging and nuclear segmentation analysis protocol for cleared 3D culture models
Imaging and subsequent segmentation analysis in three-dimensional (3D) culture models are complicated by the light scattering that occurs when collecting fluorescent signal through multiple cell and extracellular matrix layers. For 3D cell culture models to be usable for drug discovery, effective an...
Autores principales: | Boutin, Molly E., Voss, Ty C., Titus, Steven A., Cruz-Gutierrez, Kennie, Michael, Sam, Ferrer, Marc |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057966/ https://www.ncbi.nlm.nih.gov/pubmed/30042482 http://dx.doi.org/10.1038/s41598-018-29169-0 |
Ejemplares similares
-
High throughput 3D gel-based neural organotypic model for cellular assays using fluorescence biosensors
por: Kundu, Srikanya, et al.
Publicado: (2022) -
Identification by high-throughput imaging of the histone methyltransferase EHMT2 as an epigenetic regulator of VEGFA alternative splicing
por: Salton, Maayan, et al.
Publicado: (2014) -
Identification of Mammalian Protein Quality Control Factors by High-Throughput Cellular Imaging
por: Pegoraro, Gianluca, et al.
Publicado: (2012) -
HIV Nuclear Entry: Clearing the Fog
por: Shah, Vaibhav B., et al.
Publicado: (2010) -
The Treatment of Toxic Goitre with Thiouracil
por: Kennie, Agnes T.
Publicado: (1945)