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Rapid sample preparation with Lyse-It® for Listeria monocytogenes and Vibrio cholerae

Sample preparation is a leading bottleneck in rapid detection of pathogenic bacteria. Here, we use Lyse-It(®) for bacterial cellular lysis, genomic DNA fragmentation, and protein release and degradation for both Listeria monocytogenes and Vibrio cholerae. The concept of Lyse-It(®) employs a conventi...

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Detalles Bibliográficos
Autores principales: Santaus, Tonya M., Li, Shan, Ladd, Paula, Harvey, Amanda, Cole, Shannon, Stine, O. Colin, Geddes, Chris D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6059484/
https://www.ncbi.nlm.nih.gov/pubmed/30044836
http://dx.doi.org/10.1371/journal.pone.0201070