Cargando…

Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila

Pontin (Pont), also known as Tip49, encodes a member of the AAA+ (ATPases Associated with Diverse Cellular Activities) superfamily and plays pivotal roles in cell proliferation and growth, yet its function in cell death has remained poorly understood. Here we performed a genetic screen for dominant...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Xingjun, Huang, Xirui, Wu, Chenxi, Xue, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6060144/
https://www.ncbi.nlm.nih.gov/pubmed/30062057
http://dx.doi.org/10.1038/s41420-018-0074-1
_version_ 1783341977787432960
author Wang, Xingjun
Huang, Xirui
Wu, Chenxi
Xue, Lei
author_facet Wang, Xingjun
Huang, Xirui
Wu, Chenxi
Xue, Lei
author_sort Wang, Xingjun
collection PubMed
description Pontin (Pont), also known as Tip49, encodes a member of the AAA+ (ATPases Associated with Diverse Cellular Activities) superfamily and plays pivotal roles in cell proliferation and growth, yet its function in cell death has remained poorly understood. Here we performed a genetic screen for dominant modifiers of Eiger-induced JNK-dependent cell death in Drosophila, and identified Pont as a negative regulator of JNK-mediated cell death. In addition, loss of function of Pont is sufficient to induce cell death and activate the transcription of JNK target gene puc. Furthermore, the epistasis analysis indicates that Pont acts downstream of Hep. Finally, we found that Pont is also required for JNK-mediated thorax development and acts as a negative regulator of JNK phosphorylation. Together, our data suggest that pont encodes a negative component of Egr/JNK signaling pathway in Drosophila through negatively regulating JNK phosphorylation, which provides a novel role of ATPase in Egr-JNK signaling.
format Online
Article
Text
id pubmed-6060144
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-60601442018-07-30 Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila Wang, Xingjun Huang, Xirui Wu, Chenxi Xue, Lei Cell Death Discov Article Pontin (Pont), also known as Tip49, encodes a member of the AAA+ (ATPases Associated with Diverse Cellular Activities) superfamily and plays pivotal roles in cell proliferation and growth, yet its function in cell death has remained poorly understood. Here we performed a genetic screen for dominant modifiers of Eiger-induced JNK-dependent cell death in Drosophila, and identified Pont as a negative regulator of JNK-mediated cell death. In addition, loss of function of Pont is sufficient to induce cell death and activate the transcription of JNK target gene puc. Furthermore, the epistasis analysis indicates that Pont acts downstream of Hep. Finally, we found that Pont is also required for JNK-mediated thorax development and acts as a negative regulator of JNK phosphorylation. Together, our data suggest that pont encodes a negative component of Egr/JNK signaling pathway in Drosophila through negatively regulating JNK phosphorylation, which provides a novel role of ATPase in Egr-JNK signaling. Nature Publishing Group UK 2018-07-09 /pmc/articles/PMC6060144/ /pubmed/30062057 http://dx.doi.org/10.1038/s41420-018-0074-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Xingjun
Huang, Xirui
Wu, Chenxi
Xue, Lei
Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila
title Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila
title_full Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila
title_fullStr Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila
title_full_unstemmed Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila
title_short Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila
title_sort pontin/tip49 negatively regulates jnk-mediated cell death in drosophila
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6060144/
https://www.ncbi.nlm.nih.gov/pubmed/30062057
http://dx.doi.org/10.1038/s41420-018-0074-1
work_keys_str_mv AT wangxingjun pontintip49negativelyregulatesjnkmediatedcelldeathindrosophila
AT huangxirui pontintip49negativelyregulatesjnkmediatedcelldeathindrosophila
AT wuchenxi pontintip49negativelyregulatesjnkmediatedcelldeathindrosophila
AT xuelei pontintip49negativelyregulatesjnkmediatedcelldeathindrosophila