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Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila
Pontin (Pont), also known as Tip49, encodes a member of the AAA+ (ATPases Associated with Diverse Cellular Activities) superfamily and plays pivotal roles in cell proliferation and growth, yet its function in cell death has remained poorly understood. Here we performed a genetic screen for dominant...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6060144/ https://www.ncbi.nlm.nih.gov/pubmed/30062057 http://dx.doi.org/10.1038/s41420-018-0074-1 |
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author | Wang, Xingjun Huang, Xirui Wu, Chenxi Xue, Lei |
author_facet | Wang, Xingjun Huang, Xirui Wu, Chenxi Xue, Lei |
author_sort | Wang, Xingjun |
collection | PubMed |
description | Pontin (Pont), also known as Tip49, encodes a member of the AAA+ (ATPases Associated with Diverse Cellular Activities) superfamily and plays pivotal roles in cell proliferation and growth, yet its function in cell death has remained poorly understood. Here we performed a genetic screen for dominant modifiers of Eiger-induced JNK-dependent cell death in Drosophila, and identified Pont as a negative regulator of JNK-mediated cell death. In addition, loss of function of Pont is sufficient to induce cell death and activate the transcription of JNK target gene puc. Furthermore, the epistasis analysis indicates that Pont acts downstream of Hep. Finally, we found that Pont is also required for JNK-mediated thorax development and acts as a negative regulator of JNK phosphorylation. Together, our data suggest that pont encodes a negative component of Egr/JNK signaling pathway in Drosophila through negatively regulating JNK phosphorylation, which provides a novel role of ATPase in Egr-JNK signaling. |
format | Online Article Text |
id | pubmed-6060144 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60601442018-07-30 Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila Wang, Xingjun Huang, Xirui Wu, Chenxi Xue, Lei Cell Death Discov Article Pontin (Pont), also known as Tip49, encodes a member of the AAA+ (ATPases Associated with Diverse Cellular Activities) superfamily and plays pivotal roles in cell proliferation and growth, yet its function in cell death has remained poorly understood. Here we performed a genetic screen for dominant modifiers of Eiger-induced JNK-dependent cell death in Drosophila, and identified Pont as a negative regulator of JNK-mediated cell death. In addition, loss of function of Pont is sufficient to induce cell death and activate the transcription of JNK target gene puc. Furthermore, the epistasis analysis indicates that Pont acts downstream of Hep. Finally, we found that Pont is also required for JNK-mediated thorax development and acts as a negative regulator of JNK phosphorylation. Together, our data suggest that pont encodes a negative component of Egr/JNK signaling pathway in Drosophila through negatively regulating JNK phosphorylation, which provides a novel role of ATPase in Egr-JNK signaling. Nature Publishing Group UK 2018-07-09 /pmc/articles/PMC6060144/ /pubmed/30062057 http://dx.doi.org/10.1038/s41420-018-0074-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Wang, Xingjun Huang, Xirui Wu, Chenxi Xue, Lei Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila |
title | Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila |
title_full | Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila |
title_fullStr | Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila |
title_full_unstemmed | Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila |
title_short | Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila |
title_sort | pontin/tip49 negatively regulates jnk-mediated cell death in drosophila |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6060144/ https://www.ncbi.nlm.nih.gov/pubmed/30062057 http://dx.doi.org/10.1038/s41420-018-0074-1 |
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