Introducing structure-switching functionality into small-molecule-binding aptamers via nuclease-directed truncation

We report a broadly applicable enzyme digestion strategy for introducing structure-switching functionality into small-molecule-binding aptamers. This procedure is based on our discovery that exonuclease III (Exo III) digestion of aptamers is greatly inhibited by target binding. As a demonstration, w...

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Detalles Bibliográficos
Autores principales: Wang, Zongwen, Yu, Haixiang, Canoura, Juan, Liu, Yingzhu, Alkhamis, Obtin, Fu, Fengfu, Xiao, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6061857/
https://www.ncbi.nlm.nih.gov/pubmed/29718419
http://dx.doi.org/10.1093/nar/gky305

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6061857/
https://www.ncbi.nlm.nih.gov/pubmed/29718419
http://dx.doi.org/10.1093/nar/gky305

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