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Introducing structure-switching functionality into small-molecule-binding aptamers via nuclease-directed truncation
We report a broadly applicable enzyme digestion strategy for introducing structure-switching functionality into small-molecule-binding aptamers. This procedure is based on our discovery that exonuclease III (Exo III) digestion of aptamers is greatly inhibited by target binding. As a demonstration, w...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6061857/ https://www.ncbi.nlm.nih.gov/pubmed/29718419 http://dx.doi.org/10.1093/nar/gky305 |