Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells

Genome-wide screens in Drosophila cells have offered numerous insights into gene function, yet a major limitation has been the inability to stably deliver large multiplexed DNA libraries to cultured cells allowing barcoded pooled screens. Here, we developed a site-specific integration strategy for l...

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Detalles Bibliográficos
Autores principales: Viswanatha, Raghuvir, Li, Zhongchi, Hu, Yanhui, Perrimon, Norbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Chromosomes and Gene Expression
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063728/
https://www.ncbi.nlm.nih.gov/pubmed/30051818
http://dx.doi.org/10.7554/eLife.36333

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063728/
https://www.ncbi.nlm.nih.gov/pubmed/30051818
http://dx.doi.org/10.7554/eLife.36333

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