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Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells
Genome-wide screens in Drosophila cells have offered numerous insights into gene function, yet a major limitation has been the inability to stably deliver large multiplexed DNA libraries to cultured cells allowing barcoded pooled screens. Here, we developed a site-specific integration strategy for l...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
eLife Sciences Publications, Ltd
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063728/ https://www.ncbi.nlm.nih.gov/pubmed/30051818 http://dx.doi.org/10.7554/eLife.36333 |