Validating Antibodies for Quantitative Western Blot Measurements with Microwestern Array

Fluorescence-based western blots are quantitative in principal, but require determining linear range for each antibody. Here, we use microwestern array to rapidly evaluate suitable conditions for quantitative western blotting, with up to 192 antibody/dilution/replicate combinations on a single stand...

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Detalles Bibliográficos
Autores principales: Koch, Rick J., Barrette, Anne Marie, Stern, Alan D., Hu, Bin, Bouhaddou, Mehdi, Azeloglu, Evren U., Iyengar, Ravi, Birtwistle, Marc R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063895/
https://www.ncbi.nlm.nih.gov/pubmed/30054510
http://dx.doi.org/10.1038/s41598-018-29436-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063895/
https://www.ncbi.nlm.nih.gov/pubmed/30054510
http://dx.doi.org/10.1038/s41598-018-29436-0

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