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Ethylene –dependent and –independent superficial scald resistance mechanisms in ‘Granny Smith’ apple fruit
Superficial scald is a major physiological disorder of apple fruit (Malus domestica Borkh.) characterized by skin browning following cold storage; however, knowledge regarding the downstream processes that modulate scald phenomenon is unclear. To gain insight into the mechanisms underlying scald res...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6065312/ https://www.ncbi.nlm.nih.gov/pubmed/30061655 http://dx.doi.org/10.1038/s41598-018-29706-x |
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author | Karagiannis, Evangelos Michailidis, Michail Tanou, Georgia Samiotaki, Martina Karamanoli, Katerina Avramidou, Evangelia Ganopoulos, Ioannis Madesis, Panagiotis Molassiotis, Athanassios |
author_facet | Karagiannis, Evangelos Michailidis, Michail Tanou, Georgia Samiotaki, Martina Karamanoli, Katerina Avramidou, Evangelia Ganopoulos, Ioannis Madesis, Panagiotis Molassiotis, Athanassios |
author_sort | Karagiannis, Evangelos |
collection | PubMed |
description | Superficial scald is a major physiological disorder of apple fruit (Malus domestica Borkh.) characterized by skin browning following cold storage; however, knowledge regarding the downstream processes that modulate scald phenomenon is unclear. To gain insight into the mechanisms underlying scald resistance, ‘Granny Smith’ apples after harvest were treated with diphenylamine (DPA) or 1-methylcyclopropene (1-MCP), then cold stored (0 °C for 3 months) and subsequently were ripened at room temperature (20 °C for 8 days). Phenotypic and physiological data indicated that both chemical treatments induced scald resistance while 1-MCP inhibited the ethylene-dependent ripening. A combination of multi-omic analysis in apple skin tissue enabled characterization of potential genes, proteins and metabolites that were regulated by DPA and 1-MCP at pro-symptomatic and scald-symptomatic period. Specifically, we characterized strata of scald resistance responses, among which we focus on selected pathways including dehydroabietic acid biosynthesis and UDP-D-glucose regulation. Through this approach, we revealed scald-associated transcriptional, proteomic and metabolic signatures and identified pathways modulated by the common or distinct functions of DPA and 1-MCP. Also, evidence is presented supporting that cytosine methylation-based epigenetic regulation is involved in scald resistance. Results allow a greater comprehension of the ethylene–dependent and –independent metabolic events controlling scald resistance. |
format | Online Article Text |
id | pubmed-6065312 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60653122018-08-06 Ethylene –dependent and –independent superficial scald resistance mechanisms in ‘Granny Smith’ apple fruit Karagiannis, Evangelos Michailidis, Michail Tanou, Georgia Samiotaki, Martina Karamanoli, Katerina Avramidou, Evangelia Ganopoulos, Ioannis Madesis, Panagiotis Molassiotis, Athanassios Sci Rep Article Superficial scald is a major physiological disorder of apple fruit (Malus domestica Borkh.) characterized by skin browning following cold storage; however, knowledge regarding the downstream processes that modulate scald phenomenon is unclear. To gain insight into the mechanisms underlying scald resistance, ‘Granny Smith’ apples after harvest were treated with diphenylamine (DPA) or 1-methylcyclopropene (1-MCP), then cold stored (0 °C for 3 months) and subsequently were ripened at room temperature (20 °C for 8 days). Phenotypic and physiological data indicated that both chemical treatments induced scald resistance while 1-MCP inhibited the ethylene-dependent ripening. A combination of multi-omic analysis in apple skin tissue enabled characterization of potential genes, proteins and metabolites that were regulated by DPA and 1-MCP at pro-symptomatic and scald-symptomatic period. Specifically, we characterized strata of scald resistance responses, among which we focus on selected pathways including dehydroabietic acid biosynthesis and UDP-D-glucose regulation. Through this approach, we revealed scald-associated transcriptional, proteomic and metabolic signatures and identified pathways modulated by the common or distinct functions of DPA and 1-MCP. Also, evidence is presented supporting that cytosine methylation-based epigenetic regulation is involved in scald resistance. Results allow a greater comprehension of the ethylene–dependent and –independent metabolic events controlling scald resistance. Nature Publishing Group UK 2018-07-30 /pmc/articles/PMC6065312/ /pubmed/30061655 http://dx.doi.org/10.1038/s41598-018-29706-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Karagiannis, Evangelos Michailidis, Michail Tanou, Georgia Samiotaki, Martina Karamanoli, Katerina Avramidou, Evangelia Ganopoulos, Ioannis Madesis, Panagiotis Molassiotis, Athanassios Ethylene –dependent and –independent superficial scald resistance mechanisms in ‘Granny Smith’ apple fruit |
title | Ethylene –dependent and –independent superficial scald resistance mechanisms in ‘Granny Smith’ apple fruit |
title_full | Ethylene –dependent and –independent superficial scald resistance mechanisms in ‘Granny Smith’ apple fruit |
title_fullStr | Ethylene –dependent and –independent superficial scald resistance mechanisms in ‘Granny Smith’ apple fruit |
title_full_unstemmed | Ethylene –dependent and –independent superficial scald resistance mechanisms in ‘Granny Smith’ apple fruit |
title_short | Ethylene –dependent and –independent superficial scald resistance mechanisms in ‘Granny Smith’ apple fruit |
title_sort | ethylene –dependent and –independent superficial scald resistance mechanisms in ‘granny smith’ apple fruit |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6065312/ https://www.ncbi.nlm.nih.gov/pubmed/30061655 http://dx.doi.org/10.1038/s41598-018-29706-x |
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