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A highly specific SpCas9 variant is identified by in vivo screening in yeast

Despite the utility of CRISPR-Cas9 nucleases for genome editing, the potential for off-target activity limits their application, especially for therapeutic purposes1,2. We developed a yeast-based assay to identify optimized Streptococcus pyogenes Cas9 (SpCas9) variants that enables simultaneous eval...

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Detalles Bibliográficos
Autores principales: Casini, Antonio, Olivieri, Michele, Petris, Gianluca, Montagna, Claudia, Reginato, Giordano, Maule, Giulia, Lorenzin, Francesca, Prandi, Davide, Romanel, Alessandro, Demichelis, Francesca, Inga, Alberto, Cereseto, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066108/
https://www.ncbi.nlm.nih.gov/pubmed/29431739
http://dx.doi.org/10.1038/nbt.4066