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Sensitive Detection of Escherichia coli O157:H7 in Food Products by Impedimetric Immunosensors
In this work, the development of an impedimetric label-free immunosensor for the detection of Escherichia coli O157:H7 is reported. Different immobilization techniques of monoclonal anti-E. coli were tested, in order to reach the very low limit of detections. The comparison between the immobilizatio...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6068834/ https://www.ncbi.nlm.nih.gov/pubmed/29976909 http://dx.doi.org/10.3390/s18072168 |
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author | Malvano, Francesca Pilloton, Roberto Albanese, Donatella |
author_facet | Malvano, Francesca Pilloton, Roberto Albanese, Donatella |
author_sort | Malvano, Francesca |
collection | PubMed |
description | In this work, the development of an impedimetric label-free immunosensor for the detection of Escherichia coli O157:H7 is reported. Different immobilization techniques of monoclonal anti-E. coli were tested, in order to reach the very low limit of detections. The comparison between the immobilization procedures underlined the advantages of the oriented procedure and the use of a dendrimer, which allowed for immobilizing a higher number of antibody units, reaching a very high sensitivity. However, the use of activated ferrocene as electron-transferring mediator, which improved the electrical properties of the system, resulted in a very low limit of detection equal to 3 cfu/mL. This immunosensor was used to analyze milk and meat samples obtaining a good agreement with the results of the ELISA methods. |
format | Online Article Text |
id | pubmed-6068834 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60688342018-08-07 Sensitive Detection of Escherichia coli O157:H7 in Food Products by Impedimetric Immunosensors Malvano, Francesca Pilloton, Roberto Albanese, Donatella Sensors (Basel) Article In this work, the development of an impedimetric label-free immunosensor for the detection of Escherichia coli O157:H7 is reported. Different immobilization techniques of monoclonal anti-E. coli were tested, in order to reach the very low limit of detections. The comparison between the immobilization procedures underlined the advantages of the oriented procedure and the use of a dendrimer, which allowed for immobilizing a higher number of antibody units, reaching a very high sensitivity. However, the use of activated ferrocene as electron-transferring mediator, which improved the electrical properties of the system, resulted in a very low limit of detection equal to 3 cfu/mL. This immunosensor was used to analyze milk and meat samples obtaining a good agreement with the results of the ELISA methods. MDPI 2018-07-05 /pmc/articles/PMC6068834/ /pubmed/29976909 http://dx.doi.org/10.3390/s18072168 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Malvano, Francesca Pilloton, Roberto Albanese, Donatella Sensitive Detection of Escherichia coli O157:H7 in Food Products by Impedimetric Immunosensors |
title | Sensitive Detection of Escherichia coli O157:H7 in Food Products by Impedimetric Immunosensors |
title_full | Sensitive Detection of Escherichia coli O157:H7 in Food Products by Impedimetric Immunosensors |
title_fullStr | Sensitive Detection of Escherichia coli O157:H7 in Food Products by Impedimetric Immunosensors |
title_full_unstemmed | Sensitive Detection of Escherichia coli O157:H7 in Food Products by Impedimetric Immunosensors |
title_short | Sensitive Detection of Escherichia coli O157:H7 in Food Products by Impedimetric Immunosensors |
title_sort | sensitive detection of escherichia coli o157:h7 in food products by impedimetric immunosensors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6068834/ https://www.ncbi.nlm.nih.gov/pubmed/29976909 http://dx.doi.org/10.3390/s18072168 |
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