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Somatostatin Analogue Treatment Primarily Induce miRNA Expression Changes and Up-Regulates Growth Inhibitory miR-7 and miR-148a in Neuroendocrine Cells

Somatostatin (SST) analogues are used to control the proliferation and symptoms of neuroendocrine tumors (NETs). MicroRNAs (miRNA) are small non-coding RNAs that modulate posttranscriptional gene expression. We wanted to characterize the miRNAs operating under the control of SST to elucidate to what...

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Autores principales: Døssing, Kristina B. V., Kjær, Christina, Vikeså, Jonas, Binderup, Tina, Knigge, Ulrich, Culler, Michael D., Kjær, Andreas, Federspiel, Birgitte, Friis-Hansen, Lennart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070923/
https://www.ncbi.nlm.nih.gov/pubmed/29973528
http://dx.doi.org/10.3390/genes9070337
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author Døssing, Kristina B. V.
Kjær, Christina
Vikeså, Jonas
Binderup, Tina
Knigge, Ulrich
Culler, Michael D.
Kjær, Andreas
Federspiel, Birgitte
Friis-Hansen, Lennart
author_facet Døssing, Kristina B. V.
Kjær, Christina
Vikeså, Jonas
Binderup, Tina
Knigge, Ulrich
Culler, Michael D.
Kjær, Andreas
Federspiel, Birgitte
Friis-Hansen, Lennart
author_sort Døssing, Kristina B. V.
collection PubMed
description Somatostatin (SST) analogues are used to control the proliferation and symptoms of neuroendocrine tumors (NETs). MicroRNAs (miRNA) are small non-coding RNAs that modulate posttranscriptional gene expression. We wanted to characterize the miRNAs operating under the control of SST to elucidate to what extent they mediate STT actions. NCI-H727 carcinoid cell line was treated with either a chimeric SST/dopamine analogue; a SST or dopamine analogue for proliferation assays and for identifying differentially expressed miRNAs using miRNA microarray. The miRNAs induced by SST analogue treatment are investigated in carcinoid cell lines NCI-H727 and CNDT2 using in situ hybridization, qPCR and proliferation assays. SST analogues inhibited the growth of carcinoid cells more potently compared to the dopamine analogue. Principal Component Analysis (PCA) of the samples based on miRNA expression clearly separated the samples based on treatment. Two miRNAs which were highly induced by SST analogues, miR-7 and miR-148a, were shown to inhibit the proliferation of NCI-H727 and CNDT2 cells. SST analogues also produced a general up-regulation of the let-7 family members. SST analogues control and induce distinct miRNA expression patterns among which miR-7 and miR-148a both have growth inhibitory properties.
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spelling pubmed-60709232018-08-09 Somatostatin Analogue Treatment Primarily Induce miRNA Expression Changes and Up-Regulates Growth Inhibitory miR-7 and miR-148a in Neuroendocrine Cells Døssing, Kristina B. V. Kjær, Christina Vikeså, Jonas Binderup, Tina Knigge, Ulrich Culler, Michael D. Kjær, Andreas Federspiel, Birgitte Friis-Hansen, Lennart Genes (Basel) Article Somatostatin (SST) analogues are used to control the proliferation and symptoms of neuroendocrine tumors (NETs). MicroRNAs (miRNA) are small non-coding RNAs that modulate posttranscriptional gene expression. We wanted to characterize the miRNAs operating under the control of SST to elucidate to what extent they mediate STT actions. NCI-H727 carcinoid cell line was treated with either a chimeric SST/dopamine analogue; a SST or dopamine analogue for proliferation assays and for identifying differentially expressed miRNAs using miRNA microarray. The miRNAs induced by SST analogue treatment are investigated in carcinoid cell lines NCI-H727 and CNDT2 using in situ hybridization, qPCR and proliferation assays. SST analogues inhibited the growth of carcinoid cells more potently compared to the dopamine analogue. Principal Component Analysis (PCA) of the samples based on miRNA expression clearly separated the samples based on treatment. Two miRNAs which were highly induced by SST analogues, miR-7 and miR-148a, were shown to inhibit the proliferation of NCI-H727 and CNDT2 cells. SST analogues also produced a general up-regulation of the let-7 family members. SST analogues control and induce distinct miRNA expression patterns among which miR-7 and miR-148a both have growth inhibitory properties. MDPI 2018-07-04 /pmc/articles/PMC6070923/ /pubmed/29973528 http://dx.doi.org/10.3390/genes9070337 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Døssing, Kristina B. V.
Kjær, Christina
Vikeså, Jonas
Binderup, Tina
Knigge, Ulrich
Culler, Michael D.
Kjær, Andreas
Federspiel, Birgitte
Friis-Hansen, Lennart
Somatostatin Analogue Treatment Primarily Induce miRNA Expression Changes and Up-Regulates Growth Inhibitory miR-7 and miR-148a in Neuroendocrine Cells
title Somatostatin Analogue Treatment Primarily Induce miRNA Expression Changes and Up-Regulates Growth Inhibitory miR-7 and miR-148a in Neuroendocrine Cells
title_full Somatostatin Analogue Treatment Primarily Induce miRNA Expression Changes and Up-Regulates Growth Inhibitory miR-7 and miR-148a in Neuroendocrine Cells
title_fullStr Somatostatin Analogue Treatment Primarily Induce miRNA Expression Changes and Up-Regulates Growth Inhibitory miR-7 and miR-148a in Neuroendocrine Cells
title_full_unstemmed Somatostatin Analogue Treatment Primarily Induce miRNA Expression Changes and Up-Regulates Growth Inhibitory miR-7 and miR-148a in Neuroendocrine Cells
title_short Somatostatin Analogue Treatment Primarily Induce miRNA Expression Changes and Up-Regulates Growth Inhibitory miR-7 and miR-148a in Neuroendocrine Cells
title_sort somatostatin analogue treatment primarily induce mirna expression changes and up-regulates growth inhibitory mir-7 and mir-148a in neuroendocrine cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070923/
https://www.ncbi.nlm.nih.gov/pubmed/29973528
http://dx.doi.org/10.3390/genes9070337
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