Cysteine-mediated decyanation of vitamin B12 by the predicted membrane transporter BtuM

Uptake of vitamin B12 is essential for many prokaryotes, but in most cases the membrane proteins involved are yet to be identified. We present the biochemical characterization and high-resolution crystal structure of BtuM, a predicted bacterial vitamin B12 uptake system. BtuM binds vitamin B12 in it...

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Autores principales: Rempel, S., Colucci, E., de Gier, J. W., Guskov, A., Slotboom, D. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072759/
https://www.ncbi.nlm.nih.gov/pubmed/30072686
http://dx.doi.org/10.1038/s41467-018-05441-9
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author Rempel, S.
Colucci, E.
de Gier, J. W.
Guskov, A.
Slotboom, D. J.
author_facet Rempel, S.
Colucci, E.
de Gier, J. W.
Guskov, A.
Slotboom, D. J.
author_sort Rempel, S.
collection PubMed
description Uptake of vitamin B12 is essential for many prokaryotes, but in most cases the membrane proteins involved are yet to be identified. We present the biochemical characterization and high-resolution crystal structure of BtuM, a predicted bacterial vitamin B12 uptake system. BtuM binds vitamin B12 in its base-off conformation, with a cysteine residue as axial ligand of the corrin cobalt ion. Spectroscopic analysis indicates that the unusual thiolate coordination allows for decyanation of vitamin B12. Chemical modification of the substrate is a property other characterized vitamin B12-transport proteins do not exhibit.
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spelling pubmed-60727592018-08-06 Cysteine-mediated decyanation of vitamin B12 by the predicted membrane transporter BtuM Rempel, S. Colucci, E. de Gier, J. W. Guskov, A. Slotboom, D. J. Nat Commun Article Uptake of vitamin B12 is essential for many prokaryotes, but in most cases the membrane proteins involved are yet to be identified. We present the biochemical characterization and high-resolution crystal structure of BtuM, a predicted bacterial vitamin B12 uptake system. BtuM binds vitamin B12 in its base-off conformation, with a cysteine residue as axial ligand of the corrin cobalt ion. Spectroscopic analysis indicates that the unusual thiolate coordination allows for decyanation of vitamin B12. Chemical modification of the substrate is a property other characterized vitamin B12-transport proteins do not exhibit. Nature Publishing Group UK 2018-08-02 /pmc/articles/PMC6072759/ /pubmed/30072686 http://dx.doi.org/10.1038/s41467-018-05441-9 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Rempel, S.
Colucci, E.
de Gier, J. W.
Guskov, A.
Slotboom, D. J.
Cysteine-mediated decyanation of vitamin B12 by the predicted membrane transporter BtuM
title Cysteine-mediated decyanation of vitamin B12 by the predicted membrane transporter BtuM
title_full Cysteine-mediated decyanation of vitamin B12 by the predicted membrane transporter BtuM
title_fullStr Cysteine-mediated decyanation of vitamin B12 by the predicted membrane transporter BtuM
title_full_unstemmed Cysteine-mediated decyanation of vitamin B12 by the predicted membrane transporter BtuM
title_short Cysteine-mediated decyanation of vitamin B12 by the predicted membrane transporter BtuM
title_sort cysteine-mediated decyanation of vitamin b12 by the predicted membrane transporter btum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072759/
https://www.ncbi.nlm.nih.gov/pubmed/30072686
http://dx.doi.org/10.1038/s41467-018-05441-9
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