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Combined bacterial and fungal intestinal microbiota analyses: Impact of storage conditions and DNA extraction protocols

BACKGROUND: The human intestinal microbiota contains a vast community of microorganisms increasingly studied using high-throughput DNA sequencing. Standardized protocols for storage and DNA extraction from fecal samples have been established mostly for bacterial microbiota analysis. Here, we investi...

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Detalles Bibliográficos
Autores principales: Angebault, Cécile, Ghozlane, Amine, Volant, Stevenn, Botterel, Françoise, d’Enfert, Christophe, Bougnoux, Marie-Elisabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6075747/
https://www.ncbi.nlm.nih.gov/pubmed/30074988
http://dx.doi.org/10.1371/journal.pone.0201174