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Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes

The architecture of normal and diseased tissues strongly influences the development and progression of disease as well as responsiveness and resistance to therapy. We describe a tissue-based cyclic immunofluorescence (t-CyCIF) method for highly multiplexed immuno-fluorescence imaging of formalin-fix...

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Detalles Bibliográficos
Autores principales: Lin, Jia-Ren, Izar, Benjamin, Wang, Shu, Yapp, Clarence, Mei, Shaolin, Shah, Parin M, Santagata, Sandro, Sorger, Peter K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6075866/
https://www.ncbi.nlm.nih.gov/pubmed/29993362
http://dx.doi.org/10.7554/eLife.31657