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Establishing normal metabolism and differentiation in hepatocellular carcinoma cells by culturing in adult human serum
Tissue culture medium routinely contains fetal bovine serum (FBS). Here we show that culturing human hepatoma cells in their native, adult serum (human serum, HS) results in the restoration of key morphological and metabolic features of normal liver cells. When moved to HS, these cells show differen...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6076254/ https://www.ncbi.nlm.nih.gov/pubmed/30076349 http://dx.doi.org/10.1038/s41598-018-29763-2 |