High doses of CRISPR/Cas9 ribonucleoprotein efficiently induce gene knockout with low mosaicism in the hydrozoan Clytia hemisphaerica through microhomology-mediated deletion

Targeted mutagenesis using CRISPR/Cas9 technology has been shown to be a powerful approach to examine gene function in diverse metazoan species. One common drawback is that mixed genotypes, and thus variable phenotypes, arise in the F0 generation because incorrect DNA repair produces different mutat...

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Detalles Bibliográficos
Autores principales: Momose, Tsuyoshi, De Cian, Anne, Shiba, Kogiku, Inaba, Kazuo, Giovannangeli, Carine, Concordet, Jean-Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6078951/
https://www.ncbi.nlm.nih.gov/pubmed/30082705
http://dx.doi.org/10.1038/s41598-018-30188-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6078951/
https://www.ncbi.nlm.nih.gov/pubmed/30082705
http://dx.doi.org/10.1038/s41598-018-30188-0

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