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Feeding regimen modulates zebrafish behavior

Here we show that the feeding regimen modulates zebrafish (Danio rerio) behavior. With regard to the time elapsed between feeding and behavioral evaluation, fish fed 3 h before behavioral evaluation in the novel tank test (NTT) showed decreased activity and a trend toward an anxiolytic reaction (inc...

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Autores principales: Dametto, Fernanda S., Fior, Débora, Idalencio, Renan, Rosa, João Gabriel S., Fagundes, Michele, Marqueze, Alessandra, Barreto, Rodrigo E., Piato, Angelo, Barcellos, Leonardo J.G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6080598/
https://www.ncbi.nlm.nih.gov/pubmed/30090692
http://dx.doi.org/10.7717/peerj.5343
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author Dametto, Fernanda S.
Fior, Débora
Idalencio, Renan
Rosa, João Gabriel S.
Fagundes, Michele
Marqueze, Alessandra
Barreto, Rodrigo E.
Piato, Angelo
Barcellos, Leonardo J.G.
author_facet Dametto, Fernanda S.
Fior, Débora
Idalencio, Renan
Rosa, João Gabriel S.
Fagundes, Michele
Marqueze, Alessandra
Barreto, Rodrigo E.
Piato, Angelo
Barcellos, Leonardo J.G.
author_sort Dametto, Fernanda S.
collection PubMed
description Here we show that the feeding regimen modulates zebrafish (Danio rerio) behavior. With regard to the time elapsed between feeding and behavioral evaluation, fish fed 3 h before behavioral evaluation in the novel tank test (NTT) showed decreased activity and a trend toward an anxiolytic reaction (increased use of the upper section of the aquarium) in comparison to fish fed 0.5, 6, 12, 24 or 48 h before testing, although differences were not statistically significant for all comparisons. Activity and use of the upper section of the aquarium did not differ significantly among the other treatments. Regarding feeding frequency, fish fed once a day showed higher anxiety-like behavior (decreased use of the upper section of the aquarium) in comparison to fish fed twice a day, but feeding four or six times per day or only every second day did not result in differences from feeding twice a day. Feeding frequency had no effect on activity level. Metabolically, fish fed once a day presented decreased levels of glucose and glycogen and increased lactate when compared to the regular feeding (fish fed twice a day), suggesting that feeding regimen may modulate carbohydrate metabolism. Mechanistically, we suggest that the metabolic changes caused by the feeding regimen may induce behavioral changes. Our results suggest that the high variability of the results among different laboratories might be related to different feeding protocols. Therefore, if issues pertaining to the feeding regimen are not considered during experiments with zebrafish, erroneous interpretations of datasets may occur.
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spelling pubmed-60805982018-08-08 Feeding regimen modulates zebrafish behavior Dametto, Fernanda S. Fior, Débora Idalencio, Renan Rosa, João Gabriel S. Fagundes, Michele Marqueze, Alessandra Barreto, Rodrigo E. Piato, Angelo Barcellos, Leonardo J.G. PeerJ Animal Behavior Here we show that the feeding regimen modulates zebrafish (Danio rerio) behavior. With regard to the time elapsed between feeding and behavioral evaluation, fish fed 3 h before behavioral evaluation in the novel tank test (NTT) showed decreased activity and a trend toward an anxiolytic reaction (increased use of the upper section of the aquarium) in comparison to fish fed 0.5, 6, 12, 24 or 48 h before testing, although differences were not statistically significant for all comparisons. Activity and use of the upper section of the aquarium did not differ significantly among the other treatments. Regarding feeding frequency, fish fed once a day showed higher anxiety-like behavior (decreased use of the upper section of the aquarium) in comparison to fish fed twice a day, but feeding four or six times per day or only every second day did not result in differences from feeding twice a day. Feeding frequency had no effect on activity level. Metabolically, fish fed once a day presented decreased levels of glucose and glycogen and increased lactate when compared to the regular feeding (fish fed twice a day), suggesting that feeding regimen may modulate carbohydrate metabolism. Mechanistically, we suggest that the metabolic changes caused by the feeding regimen may induce behavioral changes. Our results suggest that the high variability of the results among different laboratories might be related to different feeding protocols. Therefore, if issues pertaining to the feeding regimen are not considered during experiments with zebrafish, erroneous interpretations of datasets may occur. PeerJ Inc. 2018-08-03 /pmc/articles/PMC6080598/ /pubmed/30090692 http://dx.doi.org/10.7717/peerj.5343 Text en ©2018 Dametto et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Animal Behavior
Dametto, Fernanda S.
Fior, Débora
Idalencio, Renan
Rosa, João Gabriel S.
Fagundes, Michele
Marqueze, Alessandra
Barreto, Rodrigo E.
Piato, Angelo
Barcellos, Leonardo J.G.
Feeding regimen modulates zebrafish behavior
title Feeding regimen modulates zebrafish behavior
title_full Feeding regimen modulates zebrafish behavior
title_fullStr Feeding regimen modulates zebrafish behavior
title_full_unstemmed Feeding regimen modulates zebrafish behavior
title_short Feeding regimen modulates zebrafish behavior
title_sort feeding regimen modulates zebrafish behavior
topic Animal Behavior
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6080598/
https://www.ncbi.nlm.nih.gov/pubmed/30090692
http://dx.doi.org/10.7717/peerj.5343
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