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Subcellular topography modulates actin dynamics and signaling in B-cells

B-cell signaling activation is most effectively triggered by the binding of B-cell receptors (BCRs) to membrane-bound antigens. In vivo, B-cells encounter antigen on antigen-presenting cells (APC), which possess complex surfaces with convoluted topographies, a fluid membrane and deformable cell bodi...

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Autores principales: Ketchum, Christina M., Sun, Xiaoyu, Suberi, Alexandra, Fourkas, John T., Song, Wenxia, Upadhyaya, Arpita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6080708/
https://www.ncbi.nlm.nih.gov/pubmed/29771636
http://dx.doi.org/10.1091/mbc.E17-06-0422
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author Ketchum, Christina M.
Sun, Xiaoyu
Suberi, Alexandra
Fourkas, John T.
Song, Wenxia
Upadhyaya, Arpita
author_facet Ketchum, Christina M.
Sun, Xiaoyu
Suberi, Alexandra
Fourkas, John T.
Song, Wenxia
Upadhyaya, Arpita
author_sort Ketchum, Christina M.
collection PubMed
description B-cell signaling activation is most effectively triggered by the binding of B-cell receptors (BCRs) to membrane-bound antigens. In vivo, B-cells encounter antigen on antigen-presenting cells (APC), which possess complex surfaces with convoluted topographies, a fluid membrane and deformable cell bodies. However, whether and how the physical properties of antigen presentation affect B-cell activation is not well understood. Here we use nanotopographic surfaces that allow systematic variation of geometric parameters to show that surface features on a subcellular scale influence B-cell signaling and actin dynamics. Parallel nanoridges with spacings of 3 microns or greater induce actin intensity oscillations on the ventral cell surface. Nanotopography-induced actin dynamics requires BCR signaling, actin polymerization, and myosin contractility. The topography of the stimulatory surface also modulates the distribution of BCR clusters in activated B-cells. Finally, B-cells stimulated on nanopatterned surfaces exhibit intracellular calcium oscillations with frequencies that depend on topography. Our results point to the importance of physical aspects of ligand presentation, in particular, nanotopography for B-cell activation and antigen gathering.
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spelling pubmed-60807082018-09-30 Subcellular topography modulates actin dynamics and signaling in B-cells Ketchum, Christina M. Sun, Xiaoyu Suberi, Alexandra Fourkas, John T. Song, Wenxia Upadhyaya, Arpita Mol Biol Cell Articles B-cell signaling activation is most effectively triggered by the binding of B-cell receptors (BCRs) to membrane-bound antigens. In vivo, B-cells encounter antigen on antigen-presenting cells (APC), which possess complex surfaces with convoluted topographies, a fluid membrane and deformable cell bodies. However, whether and how the physical properties of antigen presentation affect B-cell activation is not well understood. Here we use nanotopographic surfaces that allow systematic variation of geometric parameters to show that surface features on a subcellular scale influence B-cell signaling and actin dynamics. Parallel nanoridges with spacings of 3 microns or greater induce actin intensity oscillations on the ventral cell surface. Nanotopography-induced actin dynamics requires BCR signaling, actin polymerization, and myosin contractility. The topography of the stimulatory surface also modulates the distribution of BCR clusters in activated B-cells. Finally, B-cells stimulated on nanopatterned surfaces exhibit intracellular calcium oscillations with frequencies that depend on topography. Our results point to the importance of physical aspects of ligand presentation, in particular, nanotopography for B-cell activation and antigen gathering. The American Society for Cell Biology 2018-07-15 /pmc/articles/PMC6080708/ /pubmed/29771636 http://dx.doi.org/10.1091/mbc.E17-06-0422 Text en © 2018 Ketchum et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Ketchum, Christina M.
Sun, Xiaoyu
Suberi, Alexandra
Fourkas, John T.
Song, Wenxia
Upadhyaya, Arpita
Subcellular topography modulates actin dynamics and signaling in B-cells
title Subcellular topography modulates actin dynamics and signaling in B-cells
title_full Subcellular topography modulates actin dynamics and signaling in B-cells
title_fullStr Subcellular topography modulates actin dynamics and signaling in B-cells
title_full_unstemmed Subcellular topography modulates actin dynamics and signaling in B-cells
title_short Subcellular topography modulates actin dynamics and signaling in B-cells
title_sort subcellular topography modulates actin dynamics and signaling in b-cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6080708/
https://www.ncbi.nlm.nih.gov/pubmed/29771636
http://dx.doi.org/10.1091/mbc.E17-06-0422
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