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Intrinsically active MEK variants are differentially regulated by proteinases and phosphatases

MAPK/ERK kinase (MEK) 1/2 are central signaling proteins that serve as specificity determinants of the MAPK/ERK cascade. More than twenty activating mutations have been reported for MEK1/2, and many of them are known to cause diseases such as cancers, arteriovenous malformation and RASopathies. Chan...

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Autores principales: Ordan, Merav, Pallara, Chiara, Maik-Rachline, Galia, Hanoch, Tamar, Gervasio, Francesco Luigi, Glaser, Fabian, Fernandez-Recio, Juan, Seger, Rony
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6081382/
https://www.ncbi.nlm.nih.gov/pubmed/30087384
http://dx.doi.org/10.1038/s41598-018-30202-5
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author Ordan, Merav
Pallara, Chiara
Maik-Rachline, Galia
Hanoch, Tamar
Gervasio, Francesco Luigi
Glaser, Fabian
Fernandez-Recio, Juan
Seger, Rony
author_facet Ordan, Merav
Pallara, Chiara
Maik-Rachline, Galia
Hanoch, Tamar
Gervasio, Francesco Luigi
Glaser, Fabian
Fernandez-Recio, Juan
Seger, Rony
author_sort Ordan, Merav
collection PubMed
description MAPK/ERK kinase (MEK) 1/2 are central signaling proteins that serve as specificity determinants of the MAPK/ERK cascade. More than twenty activating mutations have been reported for MEK1/2, and many of them are known to cause diseases such as cancers, arteriovenous malformation and RASopathies. Changes in their intrinsic activity do not seem to correlate with the severity of the diseases. Here we studied four MEK1/2 mutations using biochemical and molecular dynamic methods. Although the studied mutants elevated the activating phosphorylation of MEK they had no effect on the stimulated ERK1/2 phosphorylation. Studying the regulatory mechanism that may explain this lack of effect, we found that one type of mutation affects MEK stability and two types of mutations demonstrate a reduced sensitivity to PP2A. Together, our results indicate that some MEK mutations exert their function not only by their elevated intrinsic activity, but also by modulation of regulatory elements such as protein stability or dephosphorylation.
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spelling pubmed-60813822018-08-10 Intrinsically active MEK variants are differentially regulated by proteinases and phosphatases Ordan, Merav Pallara, Chiara Maik-Rachline, Galia Hanoch, Tamar Gervasio, Francesco Luigi Glaser, Fabian Fernandez-Recio, Juan Seger, Rony Sci Rep Article MAPK/ERK kinase (MEK) 1/2 are central signaling proteins that serve as specificity determinants of the MAPK/ERK cascade. More than twenty activating mutations have been reported for MEK1/2, and many of them are known to cause diseases such as cancers, arteriovenous malformation and RASopathies. Changes in their intrinsic activity do not seem to correlate with the severity of the diseases. Here we studied four MEK1/2 mutations using biochemical and molecular dynamic methods. Although the studied mutants elevated the activating phosphorylation of MEK they had no effect on the stimulated ERK1/2 phosphorylation. Studying the regulatory mechanism that may explain this lack of effect, we found that one type of mutation affects MEK stability and two types of mutations demonstrate a reduced sensitivity to PP2A. Together, our results indicate that some MEK mutations exert their function not only by their elevated intrinsic activity, but also by modulation of regulatory elements such as protein stability or dephosphorylation. Nature Publishing Group UK 2018-08-07 /pmc/articles/PMC6081382/ /pubmed/30087384 http://dx.doi.org/10.1038/s41598-018-30202-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ordan, Merav
Pallara, Chiara
Maik-Rachline, Galia
Hanoch, Tamar
Gervasio, Francesco Luigi
Glaser, Fabian
Fernandez-Recio, Juan
Seger, Rony
Intrinsically active MEK variants are differentially regulated by proteinases and phosphatases
title Intrinsically active MEK variants are differentially regulated by proteinases and phosphatases
title_full Intrinsically active MEK variants are differentially regulated by proteinases and phosphatases
title_fullStr Intrinsically active MEK variants are differentially regulated by proteinases and phosphatases
title_full_unstemmed Intrinsically active MEK variants are differentially regulated by proteinases and phosphatases
title_short Intrinsically active MEK variants are differentially regulated by proteinases and phosphatases
title_sort intrinsically active mek variants are differentially regulated by proteinases and phosphatases
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6081382/
https://www.ncbi.nlm.nih.gov/pubmed/30087384
http://dx.doi.org/10.1038/s41598-018-30202-5
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