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A Novel qPCR Method for Simultaneous Detection and Quantification of Viable Pathogenic and Non-pathogenic Vibrio parahaemolyticus (tlh(+), tdh(+), and ureR(+))

Pathogenic and non-pathogenic Vibrio parahaemolyticus strains were simultaneously detected and quantified using a novel viable multiplex real-time PCR (novel qPCR). We used a new PCR primer and probe, ureR, as a surrogate for detection of the toxin trh gene as the primer was better at identifying va...

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Detalles Bibliográficos
Autores principales: Niu, Ben, Hong, Bin, Zhang, Zhaohuan, Mu, Lili, Malakar, Pradeep K., Liu, Haiquan, Pan, Yingjie, Zhao, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6083054/
https://www.ncbi.nlm.nih.gov/pubmed/30116230
http://dx.doi.org/10.3389/fmicb.2018.01747