Cargando…

Fate of the UPR marker protein Kar2/Bip and autophagic processes in fed-batch cultures of secretory insulin precursor producing Pichia pastoris

BACKGROUND: Secretory recombinant protein production with Pichia (syn. Komagataella) pastoris is commonly associated with the induction of an unfolded protein response (UPR) usually apparent through increased intracellular levels of endoplasmic reticulum (ER) resident chaperones such as Kar2/Bip. Du...

Descripción completa

Detalles Bibliográficos
Autores principales:	Roth, Gustavo, Vanz, Ana Letícia, Lünsdorf, Heinrich, Nimtz, Manfred, Rinas, Ursula
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2018
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6083527/ https://www.ncbi.nlm.nih.gov/pubmed/30092809 http://dx.doi.org/10.1186/s12934-018-0970-3

Ejemplares similares

Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures
por: Vanz, Ana Letícia, et al.
Publicado: (2014)

Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes
por: Vanz, Ana Leticia, et al.
Publicado: (2012)

Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen
por: Gurramkonda, Chandrasekhar, et al.
Publicado: (2009)

Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin
por: Gurramkonda, Chandrasekhar, et al.
Publicado: (2010)

Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen
por: Lünsdorf, Heinrich, et al.
Publicado: (2011)

Essential Roles of the Kar2/BiP Molecular Chaperone Downstream of the UPR Pathway in Cryptococcus neoformans
por: Jung, Kwang-Woo, et al.
Publicado: (2013)

Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures
por: Jahic, Mehmedalija, et al.
Publicado: (2003)

Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction
por: Resina, David, et al.
Publicado: (2007)

Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris
por: Maurer, Michael, et al.
Publicado: (2006)

A fast approach to determine a fed batch feeding profile for recombinant Pichia pastoris strains
por: Dietzsch, Christian, et al.
Publicado: (2011)

Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed-batch cultures
por: Burgard, Jonas, et al.
Publicado: (2017)

Fed-Batch Production of Saccharomyces cerevisiae L-Asparaginase II by Recombinant Pichia pastoris MUT(s) Strain
por: Rodrigues, David, et al.
Publicado: (2019)

Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate
por: Jacobs, Pieter P, et al.
Publicado: (2010)

HSF-1, HIF-1and HSP90 expression on recombinant Pichia pastoris under fed-batch fermentation
por: Zepeda, Andrea B., et al.
Publicado: (2014)

High cell density fed-batch production of insecticidal recombinant ribotoxin hirsutellin A from Pichia pastoris
por: Li, Hongbo, et al.
Publicado: (2018)

Enhancement of thermoalkaliphilic xylanase production by Pichia pastoris through novel fed-batch strategy in high cell-density fermentation
por: Shang, Tingting, et al.
Publicado: (2017)

The secretome of Pichia pastoris in fed‐batch cultivations is largely independent of the carbon source but changes quantitatively over cultivation time
por: Burgard, Jonas, et al.
Publicado: (2019)

Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch system
por: Ruottinen, Maria, et al.
Publicado: (2008)

Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase
por: Gmeiner, Christoph, et al.
Publicado: (2015)

Improving the Secretory Expression of an α-Galactosidase from Aspergillus niger in Pichia pastoris
por: Zheng, Xianliang, et al.
Publicado: (2016)

Comparison of the secretory murine DNase1 family members expressed in Pichia pastoris
por: Verhülsdonk, Lukas, et al.
Publicado: (2021)

Developing a metabolic model‐based fed‐batch feeding strategy for Pichia pastoris fermentation through fine‐tuning of the methanol utilization pathway
por: Boojari, Mohammad Amin, et al.
Publicado: (2023)

Systematic Single-Cell Analysis of Pichia pastoris Reveals Secretory Capacity Limits Productivity
por: Love, Kerry Routenberg, et al.
Publicado: (2012)

Correction: Improving the Secretory Expression of an α-Galactosidase from Aspergillus niger in Pichia pastoris
Publicado: (2016)

Efficient production of secretory Streptomyces clavuligerus β-lactamase inhibitory protein (BLIP) in Pichia pastoris
por: Law, Kin-Ho, et al.
Publicado: (2018)

New tools for high‐throughput expression of fungal secretory proteins in Saccharomyces cerevisiae and Pichia pastoris
por: González, Mario, et al.
Publicado: (2018)

Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris
por: Edwards-Jones, Bryn, et al.
Publicado: (2015)

Enhanced Production of Recombinant Secretory Proteins in Pichia pastoris by Optimizing Kex2 P1’ site
por: Yang, Song, et al.
Publicado: (2013)

Transcriptomic Analysis of Pichia pastoris (Komagataella phaffii) GS115 During Heterologous Protein Production Using a High-Cell-Density Fed-Batch Cultivation Strategy
por: Zhang, Chengbo, et al.
Publicado: (2020)

Development of quantitative metabolomics for Pichia pastoris
por: Carnicer, Marc, et al.
Publicado: (2011)

Expression of lignocellulolytic enzymes in Pichia pastoris
por: Mellitzer, Andrea, et al.
Publicado: (2012)

Dynamics in bioprocess development for Pichia pastoris
por: Spadiut, Oliver, et al.
Publicado: (2014)

Comparative genomics and transcriptomics of Pichia pastoris
por: Love, Kerry R., et al.
Publicado: (2016)

Synthetic Core Promoters for Pichia pastoris
por: Vogl, Thomas, et al.
Publicado: (2013)

Autolysis of Pichia pastoris induced by cold
por: Bartolo-Aguilar, Yaneth, et al.
Publicado: (2017)

An improved CRISPRi system in Pichia pastoris
por: Qiao, Shujing, et al.
Publicado: (2023)

Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase
por: Hemmerich, Johannes, et al.
Publicado: (2014)

Holistic process development to mitigate proteolysis of a subunit rotavirus vaccine candidate produced in Pichia pastoris by means of an acid pH pulse during fed‐batch fermentation
por: Velez‐Suberbie, M. Lourdes, et al.
Publicado: (2020)

Expression of a secretory α-glucosidase II from Apis cerana indica in Pichia pastoris and its characterization
por: Kaewmuangmoon, Jirattikarn, et al.
Publicado: (2013)

Degradation and detoxification of azo dyes with recombinant ligninolytic enzymes from Aspergillus sp. with secretory overexpression in Pichia pastoris
por: Liu, Siqi, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_v1kv7k7nmgujk559k6p25mhig5