Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS

Fast (>100 kHz) magic angle spinning solid-state NMR allows combining high-sensitive proton detection with the absence of an intrinsic molecular weight limit. Using this technique we observe for the first time narrow (1)H RNA resonances and assign nucleotide spin systems with only 200 μg of unifo...

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Detalles Bibliográficos
Autores principales: Marchanka, Alexander, Stanek, Jan, Pintacuda, Guido, Carlomagno, Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6088370/
https://www.ncbi.nlm.nih.gov/pubmed/29974085
http://dx.doi.org/10.1039/c8cc04437f
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author Marchanka, Alexander
Stanek, Jan
Pintacuda, Guido
Carlomagno, Teresa
author_facet Marchanka, Alexander
Stanek, Jan
Pintacuda, Guido
Carlomagno, Teresa
author_sort Marchanka, Alexander
collection PubMed
description Fast (>100 kHz) magic angle spinning solid-state NMR allows combining high-sensitive proton detection with the absence of an intrinsic molecular weight limit. Using this technique we observe for the first time narrow (1)H RNA resonances and assign nucleotide spin systems with only 200 μg of uniformly (13)C,(15)N-labelled RNA.
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spelling pubmed-60883702018-08-29 Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS Marchanka, Alexander Stanek, Jan Pintacuda, Guido Carlomagno, Teresa Chem Commun (Camb) Chemistry Fast (>100 kHz) magic angle spinning solid-state NMR allows combining high-sensitive proton detection with the absence of an intrinsic molecular weight limit. Using this technique we observe for the first time narrow (1)H RNA resonances and assign nucleotide spin systems with only 200 μg of uniformly (13)C,(15)N-labelled RNA. Royal Society of Chemistry 2018-08-21 2018-06-28 /pmc/articles/PMC6088370/ /pubmed/29974085 http://dx.doi.org/10.1039/c8cc04437f Text en This journal is © The Royal Society of Chemistry 2018 http://creativecommons.org/licenses/by-nc/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported Licence (CC BY-NC 3.0)
spellingShingle Chemistry
Marchanka, Alexander
Stanek, Jan
Pintacuda, Guido
Carlomagno, Teresa
Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS
title Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS
title_full Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS
title_fullStr Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS
title_full_unstemmed Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS
title_short Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS
title_sort rapid access to rna resonances by proton-detected solid-state nmr at >100 khz mas
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6088370/
https://www.ncbi.nlm.nih.gov/pubmed/29974085
http://dx.doi.org/10.1039/c8cc04437f
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