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Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue

Aberrant fibrogenesis is a feature of many diseases in multiple organ systems. The lysyl oxidase family of enzymes are central to tissue homeostasis and elevated lysyl oxidase activity is implicated in fibroproliferation as well as in cancer stroma. We have synthesised a novel fluorogenic reporter f...

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Autores principales: Aslam, Tashfeen, Miele, Amy, Chankeshwara, Sunay V., Megia-Fernandez, Alicia, Michels, Chesney, Akram, Ahsan R., McDonald, Neil, Hirani, Nik, Haslett, Chris, Bradley, Mark, Dhaliwal, Kevin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6088439/
https://www.ncbi.nlm.nih.gov/pubmed/30155003
http://dx.doi.org/10.1039/c5sc01258a
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author Aslam, Tashfeen
Miele, Amy
Chankeshwara, Sunay V.
Megia-Fernandez, Alicia
Michels, Chesney
Akram, Ahsan R.
McDonald, Neil
Hirani, Nik
Haslett, Chris
Bradley, Mark
Dhaliwal, Kevin
author_facet Aslam, Tashfeen
Miele, Amy
Chankeshwara, Sunay V.
Megia-Fernandez, Alicia
Michels, Chesney
Akram, Ahsan R.
McDonald, Neil
Hirani, Nik
Haslett, Chris
Bradley, Mark
Dhaliwal, Kevin
author_sort Aslam, Tashfeen
collection PubMed
description Aberrant fibrogenesis is a feature of many diseases in multiple organ systems. The lysyl oxidase family of enzymes are central to tissue homeostasis and elevated lysyl oxidase activity is implicated in fibroproliferation as well as in cancer stroma. We have synthesised a novel fluorogenic reporter for monitoring lysyl oxidase activity that generates a 3–5 fold increase in fluorescence following probe activation in ventilating fibrotic ex vivo asinine lung and ex vivo human lung tissue. The probe termed “oLOX” can provide real-time measurement of lysyl oxidase activity in a number of biological settings and is tractable from an in vitro setting to man.
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spelling pubmed-60884392018-08-28 Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue Aslam, Tashfeen Miele, Amy Chankeshwara, Sunay V. Megia-Fernandez, Alicia Michels, Chesney Akram, Ahsan R. McDonald, Neil Hirani, Nik Haslett, Chris Bradley, Mark Dhaliwal, Kevin Chem Sci Chemistry Aberrant fibrogenesis is a feature of many diseases in multiple organ systems. The lysyl oxidase family of enzymes are central to tissue homeostasis and elevated lysyl oxidase activity is implicated in fibroproliferation as well as in cancer stroma. We have synthesised a novel fluorogenic reporter for monitoring lysyl oxidase activity that generates a 3–5 fold increase in fluorescence following probe activation in ventilating fibrotic ex vivo asinine lung and ex vivo human lung tissue. The probe termed “oLOX” can provide real-time measurement of lysyl oxidase activity in a number of biological settings and is tractable from an in vitro setting to man. Royal Society of Chemistry 2015-08-01 2015-06-08 /pmc/articles/PMC6088439/ /pubmed/30155003 http://dx.doi.org/10.1039/c5sc01258a Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0)
spellingShingle Chemistry
Aslam, Tashfeen
Miele, Amy
Chankeshwara, Sunay V.
Megia-Fernandez, Alicia
Michels, Chesney
Akram, Ahsan R.
McDonald, Neil
Hirani, Nik
Haslett, Chris
Bradley, Mark
Dhaliwal, Kevin
Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue
title Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue
title_full Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue
title_fullStr Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue
title_full_unstemmed Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue
title_short Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue
title_sort optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6088439/
https://www.ncbi.nlm.nih.gov/pubmed/30155003
http://dx.doi.org/10.1039/c5sc01258a
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