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Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue
Aberrant fibrogenesis is a feature of many diseases in multiple organ systems. The lysyl oxidase family of enzymes are central to tissue homeostasis and elevated lysyl oxidase activity is implicated in fibroproliferation as well as in cancer stroma. We have synthesised a novel fluorogenic reporter f...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6088439/ https://www.ncbi.nlm.nih.gov/pubmed/30155003 http://dx.doi.org/10.1039/c5sc01258a |
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author | Aslam, Tashfeen Miele, Amy Chankeshwara, Sunay V. Megia-Fernandez, Alicia Michels, Chesney Akram, Ahsan R. McDonald, Neil Hirani, Nik Haslett, Chris Bradley, Mark Dhaliwal, Kevin |
author_facet | Aslam, Tashfeen Miele, Amy Chankeshwara, Sunay V. Megia-Fernandez, Alicia Michels, Chesney Akram, Ahsan R. McDonald, Neil Hirani, Nik Haslett, Chris Bradley, Mark Dhaliwal, Kevin |
author_sort | Aslam, Tashfeen |
collection | PubMed |
description | Aberrant fibrogenesis is a feature of many diseases in multiple organ systems. The lysyl oxidase family of enzymes are central to tissue homeostasis and elevated lysyl oxidase activity is implicated in fibroproliferation as well as in cancer stroma. We have synthesised a novel fluorogenic reporter for monitoring lysyl oxidase activity that generates a 3–5 fold increase in fluorescence following probe activation in ventilating fibrotic ex vivo asinine lung and ex vivo human lung tissue. The probe termed “oLOX” can provide real-time measurement of lysyl oxidase activity in a number of biological settings and is tractable from an in vitro setting to man. |
format | Online Article Text |
id | pubmed-6088439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-60884392018-08-28 Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue Aslam, Tashfeen Miele, Amy Chankeshwara, Sunay V. Megia-Fernandez, Alicia Michels, Chesney Akram, Ahsan R. McDonald, Neil Hirani, Nik Haslett, Chris Bradley, Mark Dhaliwal, Kevin Chem Sci Chemistry Aberrant fibrogenesis is a feature of many diseases in multiple organ systems. The lysyl oxidase family of enzymes are central to tissue homeostasis and elevated lysyl oxidase activity is implicated in fibroproliferation as well as in cancer stroma. We have synthesised a novel fluorogenic reporter for monitoring lysyl oxidase activity that generates a 3–5 fold increase in fluorescence following probe activation in ventilating fibrotic ex vivo asinine lung and ex vivo human lung tissue. The probe termed “oLOX” can provide real-time measurement of lysyl oxidase activity in a number of biological settings and is tractable from an in vitro setting to man. Royal Society of Chemistry 2015-08-01 2015-06-08 /pmc/articles/PMC6088439/ /pubmed/30155003 http://dx.doi.org/10.1039/c5sc01258a Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0) |
spellingShingle | Chemistry Aslam, Tashfeen Miele, Amy Chankeshwara, Sunay V. Megia-Fernandez, Alicia Michels, Chesney Akram, Ahsan R. McDonald, Neil Hirani, Nik Haslett, Chris Bradley, Mark Dhaliwal, Kevin Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue |
title | Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue
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title_full | Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue
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title_fullStr | Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue
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title_full_unstemmed | Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue
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title_short | Optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue
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title_sort | optical molecular imaging of lysyl oxidase activity – detection of active fibrogenesis in human lung tissue |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6088439/ https://www.ncbi.nlm.nih.gov/pubmed/30155003 http://dx.doi.org/10.1039/c5sc01258a |
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