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Use of Precision-Cut Lung Slices as an Ex Vivo Tool for Evaluating Viruses and Viral Vectors for Gene and Oncolytic Therapy
Organotypic slice cultures recapitulate many features of an intact organ, including cellular architecture, microenvironment, and polarity, making them an ideal tool for the ex vivo study of viruses and viral vectors. Here, we describe a procedure for generating precision-cut ovine and murine tissue...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Gene & Cell Therapy
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6092314/ https://www.ncbi.nlm.nih.gov/pubmed/30112421 http://dx.doi.org/10.1016/j.omtm.2018.07.010 |
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author | Rosales Gerpe, María C. van Vloten, Jacob P. Santry, Lisa A. de Jong, Jondavid Mould, Robert C. Pelin, Adrian Bell, John C. Bridle, Byram W. Wootton, Sarah K. |
author_facet | Rosales Gerpe, María C. van Vloten, Jacob P. Santry, Lisa A. de Jong, Jondavid Mould, Robert C. Pelin, Adrian Bell, John C. Bridle, Byram W. Wootton, Sarah K. |
author_sort | Rosales Gerpe, María C. |
collection | PubMed |
description | Organotypic slice cultures recapitulate many features of an intact organ, including cellular architecture, microenvironment, and polarity, making them an ideal tool for the ex vivo study of viruses and viral vectors. Here, we describe a procedure for generating precision-cut ovine and murine tissue slices from agarose-perfused normal and murine melanoma tumor-bearing lungs. Furthermore, we demonstrate that these precision-cut lung slices can be maintained up to 1 month and can be used for a range of applications, which include characterizing the tissue tropism of viruses that cannot be propagated in cell monolayers, evaluating the transducing properties of gene therapy vectors, and, finally, investigating the tumor specificity of oncolytic viruses. Our results suggest that ex vivo lung slices are an ideal platform for studying the tissue specificity and cancer cell selectivity of gene therapy vectors and oncolytic viruses prior to in vivo studies, providing justification for pre-clinical work. |
format | Online Article Text |
id | pubmed-6092314 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American Society of Gene & Cell Therapy |
record_format | MEDLINE/PubMed |
spelling | pubmed-60923142018-08-15 Use of Precision-Cut Lung Slices as an Ex Vivo Tool for Evaluating Viruses and Viral Vectors for Gene and Oncolytic Therapy Rosales Gerpe, María C. van Vloten, Jacob P. Santry, Lisa A. de Jong, Jondavid Mould, Robert C. Pelin, Adrian Bell, John C. Bridle, Byram W. Wootton, Sarah K. Mol Ther Methods Clin Dev Article Organotypic slice cultures recapitulate many features of an intact organ, including cellular architecture, microenvironment, and polarity, making them an ideal tool for the ex vivo study of viruses and viral vectors. Here, we describe a procedure for generating precision-cut ovine and murine tissue slices from agarose-perfused normal and murine melanoma tumor-bearing lungs. Furthermore, we demonstrate that these precision-cut lung slices can be maintained up to 1 month and can be used for a range of applications, which include characterizing the tissue tropism of viruses that cannot be propagated in cell monolayers, evaluating the transducing properties of gene therapy vectors, and, finally, investigating the tumor specificity of oncolytic viruses. Our results suggest that ex vivo lung slices are an ideal platform for studying the tissue specificity and cancer cell selectivity of gene therapy vectors and oncolytic viruses prior to in vivo studies, providing justification for pre-clinical work. American Society of Gene & Cell Therapy 2018-08-04 /pmc/articles/PMC6092314/ /pubmed/30112421 http://dx.doi.org/10.1016/j.omtm.2018.07.010 Text en © 2018 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rosales Gerpe, María C. van Vloten, Jacob P. Santry, Lisa A. de Jong, Jondavid Mould, Robert C. Pelin, Adrian Bell, John C. Bridle, Byram W. Wootton, Sarah K. Use of Precision-Cut Lung Slices as an Ex Vivo Tool for Evaluating Viruses and Viral Vectors for Gene and Oncolytic Therapy |
title | Use of Precision-Cut Lung Slices as an Ex Vivo Tool for Evaluating Viruses and Viral Vectors for Gene and Oncolytic Therapy |
title_full | Use of Precision-Cut Lung Slices as an Ex Vivo Tool for Evaluating Viruses and Viral Vectors for Gene and Oncolytic Therapy |
title_fullStr | Use of Precision-Cut Lung Slices as an Ex Vivo Tool for Evaluating Viruses and Viral Vectors for Gene and Oncolytic Therapy |
title_full_unstemmed | Use of Precision-Cut Lung Slices as an Ex Vivo Tool for Evaluating Viruses and Viral Vectors for Gene and Oncolytic Therapy |
title_short | Use of Precision-Cut Lung Slices as an Ex Vivo Tool for Evaluating Viruses and Viral Vectors for Gene and Oncolytic Therapy |
title_sort | use of precision-cut lung slices as an ex vivo tool for evaluating viruses and viral vectors for gene and oncolytic therapy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6092314/ https://www.ncbi.nlm.nih.gov/pubmed/30112421 http://dx.doi.org/10.1016/j.omtm.2018.07.010 |
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